製品の概要

  • 製品名Anti-GSK3 beta antibody [3D10]
    GSK3 beta 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3D10] to GSK3 beta
  • アプリケーション適用あり: Flow Cyt, WB, ELISA, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Monkey
  • 免疫原

    Purified recombinant C-terminal fragment of Human GSK3 beta expressed in E. coli.

  • ポジティブ・コントロール
    • WB: A549, K562, PC-12, NIH/3T3, and HEK293 cell lysates IHC-P: Lung cancer and breast cancer tissues ICC/IF: NIH/3T3 and U251 cells

アプリケーション

Our Abpromise guarantee covers the use of ab93926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/2000. Predicted molecular weight: 47 kDa.
ELISA 1/10000.
IHC-P 1/4000 - 1/10000.
IHC-Fr 1/4000 - 1/10000.

ターゲット情報

  • 機能Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair.
  • 組織特異性Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.
  • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Phosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216.
  • 細胞内局在Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Glycogen Synthase Kinase 3 Beta antibody
    • Glycogen synthase kinase-3 beta antibody
    • GSK 3 beta antibody
    • GSK-3 beta antibody
    • GSK3B antibody
    • GSK3B_HUMAN antibody
    • GSK3beta isoform antibody
    • Serine/threonine-protein kinase GSK3B antibody
    see all

Anti-GSK3 beta antibody [3D10] 画像

  • All lanes : Anti-GSK3 beta antibody [3D10] (ab93926) at 1/500 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH/3T3 cell lysate
    Lane 5 : HEK293 cell lysate


    Predicted band size : 47 kDa
  • ab93926, at a 1/4000 dilution, staining GSK3 beta in paraffin embedded Human lung cancer (left) and breast cancer (right) tissues by Immunohistochemistry. Detection: DAB staining.
  • ab93926, at a 1/4000 dilution, staining GSK3 beta in NIH/3T3 cells (left) and U251 cells (right) by Immunofluorescence (green). DRAQ5 fluorescent DNA dye is blue and Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).
  • Overlay histogram showing HeLa cells stained with ab93926 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab93926, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-GSK3 beta antibody [3D10] (ab93926) 使用論文

This product has been referenced in:
  • Rui Y  et al. Activity-dependent regulation of dendritic growth and maintenance by glycogen synthase kinase 3ß. Nat Commun 4:2628 (2013). Read more (PubMed: 24165455) »
  • Dumont M  et al. Bezafibrate administration improves behavioral deficits and tau pathology in P301S mice. Hum Mol Genet 21:5091-105 (2012). WB ; Mouse . Read more (PubMed: 22922230) »

See all 3 Publications for this product

Product Wall

If your customer wants to detect the activated form of this enzyme, the ab75745 Anti-GSK3 beta (phospho Y216) antibodyis the right choice for her: According to SwissProt, it is activated by phosphorylation at Tyr-216.


The ab93926 Anti-GSK...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"