製品の概要

  • 製品名Anti-GSK3 alpha (phospho S21) antibody
    GSK3 alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GSK3 alpha (phospho S21)
  • アプリケーション適用あり: ICC/IF, ELISA, WB, IHC-P, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    The antiserum was produced against synthesized phosphopeptide derived from human GSK3a around the phosphorylation site of Serine 21.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 5 µg/ml.
ELISA Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 50 kDa.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

ターゲット情報

Anti-GSK3 alpha (phospho S21) antibody 画像

  • Immunohistochemical analysis of paraffin embedded breast carcinoma, using primary CDK2 (Phospho-Thr160) ab28808 at 1:100 dilution and Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) secondary antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.
  • All lanes : Anti-GSK3 alpha (phospho S21) antibody (ab28808) at 1/1000 dilution

    Lane 1 : Extracts from ovary cancer cells.
    Lane 2 : Extracts from ovary cancer cells. with synthesised non phosphopeptide at 1 µg/ml
    Lane 3 : Extracts from ovary cancer cells. with synthesised phosphopeptide at 1 µg/ml

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • ICC/IF image of ab28808 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28808, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GSK3 alpha (phospho S21) antibody (ab28808) 使用論文

This product has been referenced in:
  • Borghgraef P  et al. Increasing brain protein O-GlcNAc-ylation mitigates breathing defects and mortality of Tau.P301L mice. PLoS One 8:e84442 (2013). WB ; Mouse . Read more (PubMed: 24376810) »
  • Yamaguchi N  et al. Dysfunctional ryanodine receptor and cardiac hypertrophy: role of signaling molecules. Am J Physiol Heart Circ Physiol 300:H2187-95 (2011). WB ; Mouse . Read more (PubMed: 21421818) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"