Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) 抗体 [M132] (ab45383)
Key features and details
- Mouse monoclonal [M132] to GSK3 (alpha + beta) (phospho Y216 + Y279)
- Suitable for: WB
- Reacts with: Rabbit
- Isotype: IgG1
製品の概要
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製品名
Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [M132]
GSK3 (alpha + beta) 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [M132] to GSK3 (alpha + beta) (phospho Y216 + Y279) -
由来種
Mouse -
特異性
This antibody detects 46/50 kDa proteins corresponding to the apparent molecular mass of GSK-3ß and GSK-3a -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rabbit
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human GSK3 (alpha + beta) (phospho Y216) conjugated to keyhole limpet haemocyanin.
Database link: P49841 -
ポジティブ・コントロール
- Pervanadate treated rabbit fibroblasts and treated human SKN-SH and A431 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
M132 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab45383の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Predicted molecular weight: 52 kDa.
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特記事項 |
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WB
1/1000. Predicted molecular weight: 52 kDa. |
ターゲット情報
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関連性
Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation. -
細胞内局在
Cytoplasmic and Nuclear -
参照データベース
- Entrez Gene: 56637 Mouse
- Entrez Gene: 606496 Mouse
- Entrez Gene: 50686 Rat
- Entrez Gene: 84027 Rat
- SwissProt: Q2NL51 Mouse
- SwissProt: Q9WV60 Mouse
- SwissProt: P18265 Rat
- SwissProt: P18266 Rat
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別名
- Factor A antibody
- Glycogen synthase kinase 3 alpha antibody
- Glycogen synthase kinase 3 beta antibody
see all
画像
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Lanes 1-2 : anti-GSK-3ß
Lanes 3-4 : Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [M132] (ab45383) at 1/1000 dilution
Lanes 1 & 3 : rabbit spleen fibroblasts serum starved for 2 hrs
Lanes 2 & 4 : rabbit spleen fibroblasts treated with pervanadate (1 mM) for 30 minutes
Predicted band size: 52 kDa
Observed band size: 46,50 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab45383 は 1 報の論文で使用されています。
- Puri P et al. Proteomic Analysis of Bovine Sperm YWHA Binding Partners Identify Proteins Involved in Signaling and Metabolism. Biol Reprod : (2008). WB ; Cow . PubMed: 18753613