Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) 抗体 [EPR933Y] (ab68476)
製品の概要
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製品名Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y]
GSK3 (alpha + beta) 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [EPR933Y] to GSK3 (alpha + beta) (phospho Y216 + Y279)
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特異性This antibody detects both GSK alpha phosphorylated on tyrosine 279 and GSK3 beta phosphorylyated on tyrosine 216.
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アプリケーション適用あり: WB, IP, IHC-P, ICCmore details
適用なし: Flow Cyt -
種交差性交差種: Mouse, Rat, Human, Zebrafish
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免疫原
A synthetic phospho-peptide corresponding to residues surrounding tyrosine 279 in human GSK3 alpha and tyrosine 216 in human GSK beta.
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ポジティブ･コントロール
- WB: HEK-293 cell lysates, cell lysate from untreated and nerve growth factor-beta treated PC12 cells. IHC:human brain tissue.
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特記事項
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA -
精製度Tissue culture supernatant
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ポリ/モノモノクローナル
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クローン名EPR933Y
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アイソタイプIgG
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研究分野
アプリケーション
Our Abpromise guarantee covers the use of ab68476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/500 - 1/2000. Detects a band of approximately 47-52 kDa (predicted molecular weight: 47-52 kDa). | |
| IP | 1/40. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC | 1/250 - 1/500. |
ターゲット情報
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関連性Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
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細胞内局在Cytoplasmic and Nuclear
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参照データベース
- Entrez Gene: 2931 Human
- Entrez Gene: 2932 Human
- Entrez Gene: 56637 Mouse
- Entrez Gene: 606496 Mouse
- Entrez Gene: 50686 Rat
- Entrez Gene: 84027 Rat
- Entrez Gene: 30654 Zebrafish
- Entrez Gene: 30664 Zebrafish
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別名
- Factor A antibody
- Glycogen synthase kinase 3 alpha antibody
- Glycogen synthase kinase 3 beta antibody
see all
Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] 画像
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![Western blot - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] (ab68476)](http://a.abcam.com/ps/products/68/ab68476/Images/ab68476-271750-ab68476-WB.jpg)
Western blot - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] (ab68476)All lanes : Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] (ab68476) at 1/500 dilution
Lane 1 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) treated with nerve growth factor at 100ng/ml for 5 minutes. Whole cell lysates.
Lane 3 : SH-SY5Y (Human neuroblastoma epithelial cell) treated with nerve growth factor at 100ng/ml for 5 minutes. Whole cell lysates. Then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size : 47-52 kDa
Observed band size : 47-52 kDa
Exposure time : 10 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GSK3 (alpha + beta) (phospho Y279 + Y216) antibody [EPR933Y] (ab68476)](http://a.abcam.com/ps/products/68/ab68476/Images/ab68476ihc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GSK3 (alpha + beta) (phospho Y279 + Y216) antibody [EPR933Y] (ab68476)Human brain tissue stained with ab68476 at 1/100 dilution. -
![Immunoprecipitation - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] (ab68476)](http://a.abcam.com/ps/products/68/ab68476/Images/ab68476-271441-ab68476-IP.jpg)
Immunoprecipitation - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] (ab68476)GSK3 (alpha + beta) (phospho Y216 + Y279) was immunoprecipitated from 10μg HeLa (human cervix adenocarcinoma) whole cell lysate with ab68476 at 1/50 dilution (2μg in 0.35mg lysates). Western blot was performed from the immunoprecipitate using ab68476 at 1/200 dilution (9 μg/ml). VeriBlot for IP (HRP) (ab131366), was used as the secondary antibody at 1/1000 dilution.
Lane 1 (Input): HeLa (human cervix adenocarcinoma) treated with 1uM staurosporine for 4h whole cell lysate 10μg
Lane 2 (+): HeLa (human cervix adenocarcinoma) treated with 1uM staurosporine for 4h whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab68476 in HeLa (human cervix adenocarcinoma) treated with 1uM staurosporine for 4h whole cell lysateExposure Time: 30 seconds
Blocking and diluting buffer and concentration: 5% NFDM/TBST. -
![Western blot - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] (ab68476)](http://a.abcam.com/ps/products/68/ab68476/Images/ab68476-271285-ab68476-WB.jpg)
Western blot - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] (ab68476)All lanes : Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] (ab68476)
Lane 1 : PC12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 2 : Whole cell lysate from PC12 (Rat adrenal gland pheochromocytoma cell line) cells treated with nerve growth factor-ß at 100ng/ml for 5 minutes.
Lane 3 : Whole cell lysate from PC12 (Rat adrenal gland pheochromocytoma cell line) cells ) treated with nerve growth factor-ß at 100ng/ml for 5 minutes. Membrane incubated with phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG H+L (HRP))
Developed using the ECL technique
Predicted band size : 47-52 kDa
Exposure time : 3 secondsBlocking and dilution buffer: 5% NFDM/TBST
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![Dot Blot - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] (ab68476)](http://a.abcam.com/ps/products/68/ab68476/Images/ab68476-268156-ab68476Dot-blot.jpg)
Dot Blot - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] (ab68476)Dot Blot analysis of Lane 1: GSK3 (alpha + beta) (pY216 + pY279) phospho peptide and Lane 2: GSK3 (alpha + beta) non-phospho peptide labeling GSK3 (alpha + beta) (phospho Y216 + Y279) with ab68476 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.
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![Western blot - GSK3 (alpha + beta) (phospho Y279 + Y216) antibody [EPR933Y] (ab68476)](http://a.abcam.com/ps/products/68/ab68476/Images/ab68476wb.jpg)
Western blot - GSK3 (alpha + beta) (phospho Y279 + Y216) antibody [EPR933Y] (ab68476)All lanes : Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] (ab68476) at 1/2000 dilution
Lane 1 : 293 cell lysate, cells untreated.
Lane 2 : 293 cell lysate, cells treated with AP.
Lysates/proteins at 10 µg per lane.
Secondary
HRP conjugated Goat anti-rabbit at 1/2000 dilution
Predicted band size : 47-52 kDa
Observed band size : 47-52 kDa
プロトコール
Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] (ab68476) 使用論文
This product has been referenced in:
- Xu R et al. The protease Omi regulates mitochondrial biogenesis through the GSK3ß/PGC-1a pathway. Cell Death Dis 5:e1373 (2014). WB ; Mouse . Read more (PubMed: 25118933) »
- Weigand S et al. Global quantitative phosphoproteome analysis of human tumor xenografts treated with a CD44 antagonist. Cancer Res 72:4329-39 (2012). Read more (PubMed: 22777824) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"