製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GSC2
  • 特異性
    ab65032 detects endogenous levels of total GSC2 protein.
  • アプリケーション
    適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide derived from an internal sequence of human GSC2.

  • ポジティブ・コントロール
    • 293, HepG2 and COLO205 cell extracts.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab65032 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab65032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 22 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 関連性
    GSC2, also known as Goosecoidlike or GSCL, is a homeodomain-containing gene that resides in the critical region for VCFS/DGS on 22q11. Velocardiofacial syndrome (VCFS) is a developmental disorder characterized by conotruncal heart defects, craniofacial anomalies, and learning disabilities and is phenotypically related to DiGeorge syndrome (DGS), both of which are associated with hemizygous 22q11 deletions.
  • 細胞内局在
    Nuclear
  • 参照データベース
  • 別名
    • GSC 2 antibody
    • GSCL antibody
    • Homeobox protein goosecoid 2 antibody
    • Homeobox protein goosecoid like antibody

画像

  • All lanes : Anti-GSC2 antibody (ab65032) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : HepG2 cell extract
    Lane 3 : COLO205 cell extract
    Lane 4 : COLO205 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 22 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa,34 kDa. We are unsure as to the identity of these extra bands.
  • Immunostaining of mouse E9.5 embryo tissue sections. After using a biotinylated secondary, ABC and then DAB was used for color development. Please see abreview for protocol details.

    See Abreview

参考文献

ab65032 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse ES Cells)
Loading amount
50 µg
Specification
Mouse ES Cells
Treatment
Differentiating Conditions (Minus LIF Medium)
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Utkarsh Kapoor

Verified customer

投稿 Dec 31 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (E9.5 embryo)
Specification
E9.5 embryo
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric Acid
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Dr. Jesse Mager

Verified customer

投稿 Feb 13 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録