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RabMAb

Anti-GSC 抗体 [EPR4847] (ab109024)

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製品の概要

  • 製品名
    Anti-GSC antibody [EPR4847]
    GSC 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4847] to GSC
  • 由来種
    Rabbit
  • 特異性
    Negative WB result with mouse brain, heart, kidney and spleen tissue lysates, but there is a feedback this antibody detected mouse GSC with NMuMG (mouse mammary gland, normal epithelial) cell lysate. Other samples has not been tested.
  • アプリケーション
    適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC or IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide within Human GSC. The exact sequence is proprietary.

  • ポジティブ･コントロール
    • Human brain, HT29, MCF7, and HepG2 lysates.
  • 特記事項

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab109024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IP 1/10 - 1/100.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC or IHC-P.

    • ターゲット情報

    • 機能
      Regulates chordin (CHRD). May play a role in spatial programing within discrete embryonic fields or lineage compartments during organogenesis. In concert with NKX3-2, plays a role in defining the structural components of the middle ear; required for the development of the entire tympanic ring.
    • 配列類似性
      Belongs to the paired homeobox family. Bicoid subfamily.
      Contains 1 homeobox DNA-binding domain.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
      • 別名
        • Goosecoid antibody
        • goosecoid homeobox antibody
        • GSC antibody
        • GSC_HUMAN antibody
        • Homeobox protein goosecoid antibody
        see all

      画像

      • Western blot - Anti-GSC antibody [EPR4847] (ab109024)
        Western blot - Anti-GSC antibody [EPR4847] (ab109024)
        All lanes : Anti-GSC antibody [EPR4847] (ab109024) at 1/1000 dilution

        Lane 1 : Human brain lysate
        Lane 2 : HT29 lysate
        Lane 3 : MCF7 lysate
        Lane 4 : HepG2 lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 28 kDa

      参考文献

      ab109024 has not yet been referenced specifically in any publications.

      ab109024 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      レビューと Q&A

      レビューを送る 質問を送る

      1 Review or Q&A

      10
      Votes: Highest First

      Western blot abreview for Anti-GSC antibody [EPR4847]

       Average
      Abreviews
      Abcam has not validated the combination of species/application used in this Abreview.
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (Mouse embryonic stem cells)
      Gel Running Conditions
      Reduced Denaturing (Bis-Tris from Invitrogen 4-12%)
      Loading amount
      18 µg
      Specification
      Mouse embryonic stem cells
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More
      abreview image
      Username

      Dr. Maud de Dieuleveult

      Verified customer

      投稿 Jul 04 2017

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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