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RabMAb

Anti-GSC 抗体 [EPR4847] (ab109024)

製品の概要

  • 製品名
    Anti-GSC antibody [EPR4847]
    GSC 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4847] to GSC
  • 特異性
    Negative WB result with mouse brain, heart, kidney and spleen tissue lysates, but there is a feedback this antibody detected mouse GSC with NMuMG (mouse mammary gland, normal epithelial) cell lysate. Other samples has not been tested.
  • アプリケーション
    適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC or IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human GSC.

  • ポジティブ・コントロール
    • Human brain, HT29, MCF7, and HepG2 lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab109024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IP 1/10 - 1/100.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • ターゲット情報

    • 機能
      Regulates chordin (CHRD). May play a role in spatial programing within discrete embryonic fields or lineage compartments during organogenesis. In concert with NKX3-2, plays a role in defining the structural components of the middle ear; required for the development of the entire tympanic ring.
    • 配列類似性
      Belongs to the paired homeobox family. Bicoid subfamily.
      Contains 1 homeobox DNA-binding domain.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Goosecoid antibody
      • goosecoid homeobox antibody
      • GSC antibody
      • GSC_HUMAN antibody
      • Homeobox protein goosecoid antibody
      see all

    Anti-GSC antibody [EPR4847] 画像

    • All lanes : Anti-GSC antibody [EPR4847] (ab109024) at 1/1000 dilution

      Lane 1 : Human brain lysate
      Lane 2 : HT29 lysate
      Lane 3 : MCF7 lysate
      Lane 4 : HepG2 lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 28 kDa

    Anti-GSC antibody [EPR4847] (ab109024) 使用論文

    ab109024 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse embryonic stem cells)
    Gel Running Conditions
    Reduced Denaturing (Bis-Tris from Invitrogen 4-12%)
    Loading amount
    18 µg
    Specification
    Mouse embryonic stem cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Miss. Maud De Dieuleveult

    Verified customer

    投稿 Jul 04 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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