製品の概要

  • 製品名Anti-GRPR antibody
    GRPR 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GRPR
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Chicken
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human GRPR.

    (Peptide available as ab39961.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: Human brain membrane lysate MDA MB 361 (human breast adenocarcinoma) whole cell lysate Caco2 (Human colonic carcinoma) whole cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab39883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 43 kDa).

ターゲット情報

  • 機能Receptor for gastrin releasing peptide (GRP). This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • 組織特異性Highly expressed in pancreas. Also expressed in stomach, adrenal cortex and brain.
  • 配列類似性Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BB2 antibody
    • Bombesin BB2 receptor antibody
    • bombesin receptor 2 antibody
    • Gastrin Releasing Peptide Receptor antibody
    • Gastrin-releasing peptide receptor antibody
    • GRP preferring bombesin receptor antibody
    • GRP R antibody
    • GRP-preferring bombesin receptor antibody
    • GRP-R antibody
    • Grp/bombesin receptor antibody
    • Grpr antibody
    • GRPR_HUMAN antibody
    see all

Anti-GRPR antibody 画像

  • All lanes : Anti-GRPR antibody (ab39883) at 1 µg/ml

    Lane 1 : Brain (Human) Membrane Lysate
    Lane 2 : MDA MB 361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : Caco 2 whole cell lysate (ab3950)

    Lysates/proteins at 20 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)
  • ab39883 staining human pancreas tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retreival in citrate buffer (pH 6) prior to blocking in sequential (prediluted) peroxidase and protein block for 45 minutes at 20°C.  The primary antibody was diluted 1/100 and incubated with the sample for 45 minutes at 20°C.  A HRP conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

Anti-GRPR antibody (ab39883) 使用論文

This product has been referenced in:
  • Tattersall M  et al. Expression of gastrin-releasing peptide is increased by prolonged stretch of human myometrium, and antagonists of its receptor inhibit contractility. J Physiol 590:2081-93 (2012). WB ; Human . Read more (PubMed: 22411014) »

See 1 Publication for this product

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Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Username

Dr. Sophie Pezet

Verified customer

投稿 Nov 22 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Purified protein (Caco-2 Cells)
Loading amount 200 µg
Specification Caco-2 Cells
Gel Running Conditions Non-reduced Non-Denaturing (Native) (12% acrylamide gel)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Tumor)
Specification Tumor
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (human (pancreas) mouse and rat (kidney))
Specification human (pancreas) mouse and rat (kidney)
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
Permeabilization No
Blocking step Sequential (prediluated) peroxidase and protein block as blocking agent for 45 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

投稿 Apr 04 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"