製品の概要

  • 製品名Anti-GRP94 antibody
    GRP94 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to GRP94
  • アプリケーション適用あり: IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Rabbit, Dog, Human, Cynomolgus Monkey, Syrian Hamster, Orangutan
  • 免疫原

    Synthetic peptide:

    C-KEGVKFDESEKTKE

    , corresponding to internal sequence amino acids 593-606 of Human GRP94

  • ポジティブ・コントロール
    • 3T3 lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 3 - 5 µg/ml.
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 92 kDa).
ELISA Use at an assay dependent dilution. Antibody detection limit dilution 1:32,000.

ターゲット情報

  • 機能Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
  • 配列類似性Belongs to the heat shock protein 90 family.
  • 細胞内局在Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
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Anti-GRP94 antibody 画像

  • Anti-GRP94 antibody (ab52031) at 0.03 µg/ml + 3T3 lysate (35µg protein in RIPA buffer).

    Predicted band size : 92 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • ab52031 staining GRP94 in human liver tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue section underwent antigen retrieval by steam in citrate buffer pH 6.0. The AP staining procedure was used.

Anti-GRP94 antibody (ab52031) 使用論文

ab52031 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (liver and heart)
Gel Running Conditions Reduced Denaturing
Loading amount 30 µg
Specification liver and heart
Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Mar 23 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"