製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to GRP94
  • アプリケーション
    適用あり: IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Rabbit, Dog, Human, Cynomolgus monkey, Syrian hamster, Orangutan
  • 免疫原

    Synthetic peptide:

    C-KEGVKFDESEKTKE

    , corresponding to internal sequence amino acids 593-606 of Human GRP94

  • ポジティブ・コントロール
    • 3T3 lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 3 - 5 µg/ml.
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 92 kDa).
ELISA Use at an assay dependent dilution. Antibody detection limit dilution 1:32,000.

ターゲット情報

  • 機能
    Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
  • 配列類似性
    Belongs to the heat shock protein 90 family.
  • 細胞内局在
    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 94 kDa glucose regulated protein antibody
    • 94 kDa glucose-regulated protein antibody
    • ECGP antibody
    • Endoplasmin antibody
    • Endothelial cell (HBMEC) glycoprotein antibody
    • ENPL_HUMAN antibody
    • Glucose regulated protein 94kDa antibody
    • gp96 antibody
    • gp96 homolog antibody
    • GRP 94 antibody
    • GRP-94 antibody
    • Heat shock protein 90 kDa beta member 1 antibody
    • heat shock protein 90kDa beta (Grp94), member 1 antibody
    • Heat shock protein, 90 kDa, beta, 1 antibody
    • HSP90B1 antibody
    • Stress inducible tumor rejection antigen GP96 antibody
    • TRA1 antibody
    • tumor rejection antigen (gp96) 1 antibody
    • Tumor rejection antigen 1 antibody
    • Tumor rejection antigen gp96 antibody
    • Tumor rejection antigen-1 (gp96) antibody
    see all

画像

  • Anti-GRP94 antibody (ab52031) at 0.03 µg/ml + 3T3 lysate (35µg protein in RIPA buffer).

    Predicted band size : 92 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • ab52031 staining GRP94 in human liver tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue section underwent antigen retrieval by steam in citrate buffer pH 6.0. The AP staining procedure was used.

参考文献

ab52031 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Rat Tissue lysate - whole (liver and heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
liver and heart
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Mar 23 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録