製品の概要

  • 製品名Anti-GRP94 antibody
    GRP94 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GRP94
  • アプリケーション適用あり: WB, ICC, IP, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Dog, Human
  • 免疫原

    Synthetic peptide corresponding to Mouse GRP94 aa 787-802 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    EEEEETEKESTEKDEL


    Database link: P08113

  • ポジティブ・コントロール
    • Rat Brain Tissue Extract.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab13509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 94 kDa. 2µg/mL of ab13509 detects of Grp94 in 20µg of Rat Brain Tissue Extract using colorimetric analysis. 1/2000 for ECL detection.
ICC Use at an assay dependent concentration.
IP Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
  • 配列類似性Belongs to the heat shock protein 90 family.
  • 細胞内局在Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 94 kDa glucose regulated protein antibody
    • 94 kDa glucose-regulated protein antibody
    • ECGP antibody
    • Endoplasmin antibody
    • Endothelial cell (HBMEC) glycoprotein antibody
    • ENPL_HUMAN antibody
    • Glucose regulated protein 94kDa antibody
    • gp96 antibody
    • gp96 homolog antibody
    • GRP 94 antibody
    • GRP-94 antibody
    • Heat shock protein 90 kDa beta member 1 antibody
    • heat shock protein 90kDa beta (Grp94), member 1 antibody
    • Heat shock protein, 90 kDa, beta, 1 antibody
    • HSP90B1 antibody
    • Stress inducible tumor rejection antigen GP96 antibody
    • TRA1 antibody
    • tumor rejection antigen (gp96) 1 antibody
    • Tumor rejection antigen 1 antibody
    • Tumor rejection antigen gp96 antibody
    • Tumor rejection antigen-1 (gp96) antibody
    see all

Anti-GRP94 antibody 画像

  • ICC/IF image of ab13509 stained rat PC12 cells. The cells were 4% PFA and 100% methanol fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13509, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
  • All lanes : Anti-GRP94 antibody (ab13509) at 1/1000 dilution

    Lane 1 : Molecular weight ladder
    Lane 2 : Lysates prepared from rat brain
    Lane 3 : Lysates prepared from mouse brain
    Lane 4 : Lysates prepared from rat liver microsomes
    Lane 5 : Lysates prepared from mouse liver microsomes
    Lane 6 : Lysates prepared from human liver microsomes


    Predicted band size : 94 kDa
  • Anti-GRP94 antibody (ab13509) at 2 µg/ml + Recombinant Human GRP94 protein (ab87144) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 150 seconds
  • GRP94 was immunoprecipitated using 0.5mg Mouse Brain tissue lysate, 5µg of Rabbit polyclonal to GRP94 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab13509.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 100kDa: GRP94

Anti-GRP94 antibody (ab13509) 使用論文

This product has been referenced in:
  • Rosenbaum M  et al. MZB1 is a GRP94 cochaperone that enables proper immunoglobulin heavy chain biosynthesis upon ER stress. Genes Dev 28:1165-78 (2014). IP ; Mouse . Read more (PubMed: 24888588) »
  • Lupachyk S  et al. Endoplasmic reticulum stress plays a key role in the pathogenesis of diabetic peripheral neuropathy. Diabetes 62:944-52 (2013). WB ; Mouse, Rat . Read more (PubMed: 23364451) »

See all 9 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"