製品の概要

  • 製品名Anti-GRP78 BiP antibody [EPR4040(2)]
    GRP78 BiP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4040(2)] to GRP78 BiP
  • アプリケーション適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human GRP78 BiP.

  • ポジティブ・コントロール
    • HeLa, LnCaP, ECV-304, C6, NIH/3T3, PC-12, and RAW264.7 and HepG2 cell lysates. Human brain and testis tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EPR4040(2)
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab108613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 78 kDa (predicted molecular weight: 72 kDa).Can be blocked with GRP78 BiP peptide (ab176143).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
  • 追加情報Is unsuitable for Flow Cyt,ICC or IP.
  • ターゲット情報

    • 機能Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
    • 関連疾患Autoantigen in rheumatoid arthritis.
    • 配列類似性Belongs to the heat shock protein 70 family.
    • 細胞内局在Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 78 kDa glucose regulated protein antibody
      • 78 kDa glucose-regulated protein antibody
      • AL022860 antibody
      • AU019543 antibody
      • BIP antibody
      • D2Wsu141e antibody
      • D2Wsu17e antibody
      • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody
      • Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody
      • Epididymis secretory sperm binding protein Li 89n antibody
      • FLJ26106 antibody
      • Glucose Regulated Protein 78kDa antibody
      • GRP 78 antibody
      • GRP-78 antibody
      • GRP78 antibody
      • GRP78_HUMAN antibody
      • Heat shock 70 kDa protein 5 antibody
      • Heat Shock 70kDa Protein 5 antibody
      • Heat shock protein family A (Hsp70) member 5 antibody
      • HEL S 89n antibody
      • Hsce70 antibody
      • HSPA 5 antibody
      • HSPA5 antibody
      • Immunoglobulin Heavy Chain Binding Protein antibody
      • Immunoglobulin heavy chain-binding protein antibody
      • mBiP antibody
      • MIF2 antibody
      • Sez7 antibody
      see all

    Anti-GRP78 BiP antibody [EPR4040(2)] 画像

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab108613 at 1/250
    • Immunohistochemical analysis of paraffin-embedded Human testis tissue using ab108613 at 1/250
    • All lanes : Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : LnCaP cell lysate
      Lane 3 : ECV-304 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 72 kDa
      Observed band size : 78 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/1000 dilution

      Lane 1 : C6 cell lysate
      Lane 2 : NIH/3T3 cell lysate
      Lane 3 : PC-12 cell lysate
      Lane 4 : RAW264.7 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 72 kDa
      Observed band size : 78 kDa (why is the actual band size different from the predicted?)

    Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) 使用論文

    This product has been referenced in:
    • Russ DW  et al. Muscle-specificity of age-related changes in markers of autophagy and sphingolipid metabolism. Biogerontology 16:747-59 (2015). WB ; Rat . Read more (PubMed: 26296420) »
    • Jones LW  et al. Modulation of circulating angiogenic factors and tumor biology by aerobic training in breast cancer patients receiving neoadjuvant chemotherapy. Cancer Prev Res (Phila) 6:925-37 (2013). Human . Read more (PubMed: 23842792) »

    See all 3 Publications for this product

    Product Wall

    Thank you for contacting us. Below is the protocol used to validate ab108613 in IHC-P. Please let me know if you need any additional information or assistance.

    1. Solutions and reagents
    1.1. Xylene
    1.2. Ethanol, anhydrous denatured...

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    "We only saw a faint band at 50 kDa using mouse and rat heart, which looks non-specific on our blots. I have not found the identity of this band in the literature. The blocking reagent used was 5% milk. We used our standard WB protocol (...

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    Thank you for your reply.
    If you were able to detect a different ER protein using the same samples then it doesn't seem like it was a problem with the sample preparation.
    I would like to offer you a free of charge replacement of ab108613 (alt...

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    Thank you for taking time to send us your protocol. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring prod...

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    Merci de votre appel. Je suis ravie que vous souhaitiez participer a notre offre et tester ab108613 en immunofluorescence sur des cellules (ICC/IF). Le code de réduction correspondant est xx. Ce code de réduction est utilisable sous 120 jours et p...

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    Thank you for contacting us, please find the requested CoC attached. Please let me know if you need any other information!

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"