製品の概要

  • 製品名Anti-Grp75 antibody
    Grp75 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Grp75
  • 特異性Detects endogenous levels of total Grp75 protein
  • アプリケーション適用あり: WB, IHC-P, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from the C-terminus of human GRP75

  • ポジティブ・コントロール
    • Human breast carinoma tissue; Cos7 cell extract

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/300 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone.
  • 配列類似性Belongs to the heat shock protein 70 family.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Mitochondrion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 75 kDa glucose regulated protein antibody
    • 75 kDa glucose-regulated protein antibody
    • CSA antibody
    • Glucose Regulated Protein antibody
    • Grp 75 antibody
    • GRP-75 antibody
    • GRP75 antibody
    • GRP75_HUMAN antibody
    • Heat shock 70 kDa protein 9 antibody
    • Heat shock 70kD protein 9 antibody
    • heat shock 70kDa protein 9 antibody
    • Heat shock 70kDa protein 9B antibody
    • Heat shock protein 74 kDa A antibody
    • Heat shock protein A antibody
    • Heat shock protein cognate 74 antibody
    • Hsc74 antibody
    • Hsp74 antibody
    • Hsp74a antibody
    • HSPA9 antibody
    • Hspa9a antibody
    • HSPA9B antibody
    • MGC4500 antibody
    • mitochondrial antibody
    • Mortalin 2 antibody
    • Mortalin antibody
    • Mortalin perinuclear antibody
    • Mortalin2 antibody
    • MOT 2 antibody
    • MOT antibody
    • MOT2 antibody
    • Mthsp70 antibody
    • p66 mortalin antibody
    • P66 MOT antibody
    • PBP74 antibody
    • Peptide binding protein 74 antibody
    • Peptide-binding protein 74 antibody
    • Stress 70 protein mitochondrial antibody
    • Stress 70 protein mitochondrial precursor antibody
    • Stress-70 protein antibody
    see all

Anti-Grp75 antibody 画像

  • Immunohistochemical analysis of GRP75 in paraffin-embedded human breast carcinoma tissue, using ab53098 at 1/50 dilution, in the presence (right) or absence (left) of peptide.
  • All lanes : Anti-Grp75 antibody (ab53098) at 1/300 dilution

    Lane 1 : Cos7 cell extract
    Lane 2 : Cos7 cell extract with peptide


    Predicted band size : 74 kDa
    Observed band size : 74 kDa
  • ICC/IF image of ab53098 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53098, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Grp75 antibody (ab53098) 使用論文

This product has been referenced in:
  • Chen J  et al. Overexpression of Mortalin in hepatocellular carcinoma and its relationship with angiogenesis and epithelial to mesenchymal transition. Int J Oncol 44:247-55 (2014). WB, IHC-P ; Human . Read more (PubMed: 24190572) »
  • Shelton MN  et al. Secretion modification region-derived peptide disrupts HIV-1 Nef's interaction with mortalin and blocks virus and Nef exosome release. J Virol 86:406-19 (2012). WB ; Human . Read more (PubMed: 22013042) »

See all 6 Publications for this product

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Thank you for your inquiry.

Please excuse the delay in my answer. Unfortunately, I cannot recommend to this antibody with drosophila after checking forthe homology of theimmunogen sequence.

The human immungen only shows a homology o...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Small intestine)
Specification Small intestine
Fixative Formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM citric acid buffer
Permeabilization No
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 20°C
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投稿 May 22 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Colonic mucosa)
Loading amount 20 µg
Specification Colonic mucosa
Treatment 6 hours following 12Gy gamma radiation
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

投稿 May 22 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"