製品の概要

  • 製品名Anti-Growth Hormone antibody [GH-45]
    Growth Hormone 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [GH-45] to Growth Hormone
  • 特異性Specifically reacts with human growth hormone with affinity constant 3.8 x 1010 l /mol. It does not bind human prolactin or any other pituitary hormones.
  • アプリケーション適用あり: ICC, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length native protein (purified) (Human).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein A purified
  • 特記事項(精製)Purified from ascites using protein A-affinity chromatography Purity >95 % (by PAGE).
  • ポリ/モノモノクローナル
  • クローン名GH-45
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC
IHC-P
  • 追加情報ICC: Use at an assay dependant dilution.
    IHC-P: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
    • 関連疾患Defects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A) [MIM:262400]; also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH.
      Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH.
      Defects in GH1 are the cause of Kowarski syndrome (KWKS) [MIM:262650]; also known as pituitary dwarfism VI.
      Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2) [MIM:173100]. IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy.
    • 配列類似性Belongs to the somatotropin/prolactin family.
    • 細胞内局在Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • gH antibody
      • GH-N antibody
      • GH1 antibody
      • GHN antibody
      • Growth hormone 1 antibody
      • Growth hormone antibody
      • Growth hormone, normal antibody
      • Growth hormone, pituitary antibody
      • HG1 antibody
      • hGH-N antibody
      • IGHD1B antibody
      • Pituitary growth hormone antibody
      • RNGHGP antibody
      • SOMA_HUMAN antibody
      • Somatotropin antibody
      see all

    Anti-Growth Hormone antibody [GH-45] 画像

    • Human normal brain (pituitary gland). Staining is observed in the cytoplasm and in the extracellulat space. Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for mouse for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

    Anti-Growth Hormone antibody [GH-45] (ab7905) 使用論文

    This product has been referenced in:
    • Ho Y  et al. Distinct chromatin configurations regulate the initiation and the maintenance of hGH gene expression. Mol Cell Biol 33:1723-34 (2013). Read more (PubMed: 23428872) »
    • Barbieri F  et al. Overexpression of stromal cell-derived factor 1 and its receptor CXCR4 induces autocrine/paracrine cell proliferation in human pituitary adenomas. Clin Cancer Res 14:5022-32 (2008). IHC-P ; Human . Read more (PubMed: 18698020) »

    See all 2 Publications for this product

    Product Wall

    I have enquired with the suppliers of the other Growth hormone antibodies that we carry, and as for ab7905 the supplier informed me that the antibody reacts with human growth hormone with affinity constant 3.8 x 10E10 l /mol. I am still waiting to he...

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    I would not recommend the use of an antibody raised in mouse for use in IHC on mouse tissue. This is because the anti-mouse secondary would also detect endogenous mouse IgGs in the tissue. ab8490 -- Rabbit polyclonal to human Growth Hormone -- has been...

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    This antibody has not been tested in rats. Currently we do not stock any other antibodies to growth hormones

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"