製品の概要

  • 製品名Anti-GRO gamma antibody
    GRO gamma 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GRO gamma
  • アプリケーション適用あり: WB, ELISA, Neutralisingmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Highly pure (>98%) recombinant hGROg (human GRO-gamma)

製品の特性

  • 製品の状態Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーPBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプunknown
  • 軽鎖の種類unknown
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab9843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
Neutralising
  • 追加情報Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hGROg (100.00 ng/ml), a concentration of 1.0 - 2.0 µg/ml of this antibody is required.

    ELISA:To detect hGROg by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hGROg.

    Western Blot:To detect hGROg by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGROg is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
  • ターゲット情報

    • 機能Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes.
    • 配列類似性Belongs to the intercrine alpha (chemokine CxC) family.
    • 翻訳後修飾N-terminal processed form GRO-gamma(5-73) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.
    • 細胞内局在Secreted.
    • Information by UniProt
    • 参照データベース
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    Anti-GRO gamma antibody 画像

    • Anti-GRO gamma antibody (ab9843) at 1 µg/ml + Recombinant Human GRO gamma protein (ab92858) at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 10 seconds

    Anti-GRO gamma antibody (ab9843) 使用論文

    ab9843 has not yet been referenced specifically in any publications.

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