Anti-GRO alpha + GRO gamma + GRO beta 抗体 [EPR4902] (ab124898)
製品の概要
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製品名Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902]
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製品の詳細Rabbit monoclonal [EPR4902] to GRO alpha + GRO gamma + GRO beta
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由来種Rabbit
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アプリケーション適用あり: WBmore details
適用なし: ICC/IF,IHC-P or IP -
種交差性交差種: Human
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免疫原
Synthetic peptide within Human GRO alpha aa 50-150. The exact sequence is proprietary.
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ポジティブ･コントロール
- Human GRO alpha recombinant protein.
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特記事項
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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バッファーpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA -
精製度Tissue culture supernatant
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ポリ/モノモノクローナル
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クローン名EPR4902
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アイソタイプIgG
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研究分野
アプリケーション
Our Abpromise guarantee covers the use of ab124898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 8 kDa (predicted molecular weight: 11 kDa). |
ターゲット情報
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細胞内局在GRO alpha: Secreted. GRO gamma: Secreted.
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参照データベース
- Entrez Gene: 2919 Human
- Entrez Gene: 2921 Human
- Omim: 139111 Human
- Omim: 155730 Human
- SwissProt: P09341 Human
- SwissProt: P19875 Human
- SwissProt: P19876 Human
- Unigene: 708652 Human
see all
画像
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![Western blot - Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902] (ab124898)](http://a.abcam.com/ps/products/124/ab124898/Images/ab124898-261062-124898.jpg)
Western blot - Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902] (ab124898)All lanes : Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902] (ab124898) at 1/2000 dilution
Lane 1 : Human GRO alpha active protein
Lane 2 : Human GRO beta fragment recombinant protein
Lane 3 : Human GRO gamma fragment recombinant protein
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1 & 3 : Goat Anti-Rabbit IgG,(H+L),Peroxidase conjugated at 1/100000 dilution
Lane 2 : Goat Ant-Rabbit IgG,(H+L),Peroxidase conjugated at 1/100000 dilution
Predicted band size: 11 kDa
Observed band size: 10,36,10 kDa (why is the actual band size different from the predicted?)Exposure time: 3 minutes
Blocking buffer and concentartion:5% NFDM/TBST
Diluting buffer and concentration:5% NFDM/TBST
Human GRO beta fragment pritein contain aa35-107 with a GST-Tage.Human GRO gamma fragment recombinant protein contain aa35-107 with a His-Tag.
プロトコール
参考文献
This product has been referenced in:
- Pasquier J et al. Circulating microparticles in acute diabetic Charcot foot exhibit a high content of inflammatory cytokines, and support monocyte-to-osteoclast cell induction. Sci Rep 7:16450 (2017). Read more (PubMed: 29180664) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"