Anti-CXCL1/GRO alpha 抗体 [MM0208-9A18] (ab89318)
Key features and details
- Mouse monoclonal [MM0208-9A18] to CXCL1/GRO alpha
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2
製品の概要
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製品名
Anti-CXCL1/GRO alpha antibody [MM0208-9A18]
CXCL1/GRO alpha 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MM0208-9A18] to CXCL1/GRO alpha -
由来種
Mouse -
特異性
Cross reacts weakly with GRO beta. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length Human protein.
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ポジティブ・コントロール
- WB: Human placenta tissue lysate. IHC-P: Human placenta tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
MM0208-9A18 -
アイソタイプ
IgG2 -
研究分野
関連製品
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Compatible Secondaries
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab89318の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 11 kDa.
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IHC-P |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 11 kDa. |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻訳後修飾
N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 2919 Human
- Omim: 155730 Human
- SwissProt: P09341 Human
- Unigene: 708652 Human
- Unigene: 789 Human
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別名
- C-X-C motif chemokine 1 antibody
- Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) antibody
- chemokine (C-X-C motif) ligand 1 antibody
see all
画像
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Anti-CXCL1/GRO alpha antibody [MM0208-9A18] (ab89318) at 1/500 dilution + Human placenta tissue lysate
Predicted band size: 11 kDa
Observed band size: 8 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4µm) labelling CXCL1/GRO alpha with ab89318. Tissue was treated with 15µg/ml Protenase K for antigen retrieval and fixed with 4% PFA. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (5)
ab89318 は 5 報の論文で使用されています。
- Zheng H et al. Integrated single-cell and bulk RNA sequencing analysis identifies a cancer associated fibroblast-related signature for predicting prognosis and therapeutic responses in colorectal cancer. Cancer Cell Int 21:552 (2021). PubMed: 34670584
- Yang C et al. CXCL1 stimulates migration and invasion in ER-negative breast cancer cells via activation of the ERK/MMP2/9 signaling axis. Int J Oncol 55:684-696 (2019). PubMed: 31322183
- Zou Y & Li L Identification of six serum antigens and autoantibodies for the detection of early stage epithelial ovarian carcinoma by bioinformatics analysis and liquid chip analysis. Oncol Lett 16:3231-3240 (2018). PubMed: 30127919
- Miyake M et al. CXCL1-Mediated Interaction of Cancer Cells with Tumor-Associated Macrophages and Cancer-Associated Fibroblasts Promotes Tumor Progression in Human Bladder Cancer. Neoplasia 18:636-646 (2016). PubMed: 27690238
- Miyake M et al. Expression of CXCL1 in human endothelial cells induces angiogenesis through the CXCR2 receptor and the ERK1/2 and EGF pathways. Lab Invest 93:768-78 (2013). PubMed: 23732813