Anti-CXCL1/GRO alpha 抗体 (ab86436)
Key features and details
- Rabbit polyclonal to CXCL1/GRO alpha
- Suitable for: WB
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-CXCL1/GRO alpha antibody
CXCL1/GRO alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CXCL1/GRO alpha -
由来種
Rabbit -
特異性
The immunogen for ab86436 has >90% homology to mouse GRO beta -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human, Recombinant fragment -
免疫原
Synthetic peptide surrounding amino acid 78 of mouse GRO alpha.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called 5212 KC Antibody.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.5% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86436の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 11 kDa).
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特記事項 |
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WB
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 11 kDa). |
ターゲット情報
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機能
Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻訳後修飾
N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 2919 Human
- Entrez Gene: 14825 Mouse
- Omim: 155730 Human
- SwissProt: P09341 Human
- SwissProt: P12850 Mouse
- Unigene: 708652 Human
- Unigene: 789 Human
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別名
- C-X-C motif chemokine 1 antibody
- Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) antibody
- chemokine (C-X-C motif) ligand 1 antibody
see all
画像
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All lanes : Anti-CXCL1/GRO alpha antibody (ab86436) at 0.5 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : 3T3 cell lysate
Predicted band size: 11 kDa
Observed band size: 9 kDa why is the actual band size different from the predicted? -
All lanes : Anti-CXCL1/GRO alpha antibody (ab86436) at 0.5 µg/ml
Lane 1 :Recombinant rat CXCL1/GRO alpha protein (ab9773) at 0.1 µg
Lane 2 :Recombinant rat CXCL1/GRO alpha protein (ab9773) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 11 kDa
Exposure time: 1 minute
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (37)
ab86436 は 37 報の論文で使用されています。
- Chang HX & Zhao YF Protocatechuic acid as an inhibitor of the JNK/CXCL1/CXCR2 pathway relieves neuropathic pain in CCI rats. Bosn J Basic Med Sci 22:217-228 (2022). PubMed: 34813418
- Saxena S et al. Differential expression profile of CXC-receptor-2 ligands as potential biomarkers in pancreatic ductal adenocarcinoma. Am J Cancer Res 12:68-90 (2022). PubMed: 35141005
- Catar RA et al. Angiogenic Role of Mesothelium-Derived Chemokine CXCL1 During Unfavorable Peritoneal Tissue Remodeling in Patients Receiving Peritoneal Dialysis as Renal Replacement Therapy. Front Immunol 13:821681 (2022). PubMed: 35185912
- Zingariello M et al. Resident Self-Tissue of Proinflammatory Cytokines Rather Than Their Systemic Levels Correlates with Development of Myelofibrosis in Gata1low Mice. Biomolecules 12:N/A (2022). PubMed: 35204735
- Wang Y et al. Gut-Liver Axis: Liver Sinusoidal Endothelial Cells Function as the Hepatic Barrier in Colitis-Induced Liver Injury. Front Cell Dev Biol 9:702890 (2021). PubMed: 34336855