製品の概要

  • 製品名Anti-GRO alpha antibody
    GRO alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GRO alpha
  • 特異性The immunogen for ab86436 has >90% homology to mouse GRO beta
  • アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide surrounding amino acid 78 of mouse GRO alpha.

  • ポジティブ・コントロール
    • Jurkat and 3T3 cell lysates.

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 30% Glycerol, 0.5% BSA, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab86436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 11 kDa).

ターゲット情報

  • 機能Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
  • 配列類似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 翻訳後修飾N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C-X-C motif chemokine 1 antibody
    • Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) antibody
    • chemokine (C-X-C motif) ligand 1 antibody
    • CINC-1 antibody
    • CXCL1 antibody
    • Cytokine-induced neutrophil chemoattractant 1 antibody
    • Fibroblast secretory protein antibody
    • Fsp antibody
    • Gro 1 antibody
    • Gro A antibody
    • Gro antibody
    • GRO protein, alpha antibody
    • GRO-alpha(1-73) antibody
    • GRO-alpha(6-73) antibody
    • Gro1 antibody
    • GRO1 oncogene (melanoma growth stimulating activity, alpha) antibody
    • GRO1 oncogene (melanoma growth-stimulating activity) antibody
    • Gro1 oncogene antibody
    • GROa antibody
    • GROA_HUMAN antibody
    • Growth-regulated alpha protein antibody
    • KC antibody
    • KC chemokine, mouse, homolog of antibody
    • melanoma growth stimulatory activity alpha antibody
    • Melanoma growth stimulatory activity antibody
    • Melanoma growth stimulatory activity, alpha antibody
    • MGSA alpha antibody
    • MGSA antibody
    • MGSA-a antibody
    • N51 antibody
    • NAP-3 antibody
    • NAP3 antibody
    • Neutrophil-activating protein 3 antibody
    • Platelet-derived growth factor-inducible protein KC antibody
    • Scyb 1 antibody
    • Scyb1 antibody
    • Secretory protein N51 antibody
    • Small inducible cytokine subfamily B, member 1 antibody
    see all

Anti-GRO alpha antibody 画像

  • All lanes : Anti-GRO alpha antibody (ab86436) at 0.5 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : 3T3 cell lysate


    Predicted band size : 11 kDa
    Observed band size : 9 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab86436 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86436, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-GRO alpha antibody (ab86436) at 0.5 µg/ml

    Lane 1 : Recombinant GRO alpha protein (ab9773) at 0.1 µg
    Lane 2 : Recombinant GRO alpha protein (ab9773) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

Anti-GRO alpha antibody (ab86436) 使用論文

This product has been referenced in:
  • Benakis C  et al. Neuroprotection by inhibiting the c-Jun N-terminal kinase pathway after cerebral ischemia occurs independently of interleukin-6 and keratinocyte-derived chemokine (KC/CXCL1) secretion. J Neuroinflammation 9:76 (2012). IHC-Fr ; Mouse . Read more (PubMed: 22533966) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human Tissue sections (Intervertebral Discs))
Specification Human Tissue sections (Intervertebral Discs)
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris Buffer pH:9,5 for 2x5’ in MW 40% power
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: 22°C
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投稿 Apr 11 2012

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I am very pleased to hear you would like to test ab86436, ab9841and ab10064with IHC ofparaffin embedded human tissue sections. The codes below will each give you 1 freeprimary antibodybefore the expiration d...

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