Anti-GRLF1 (phospho Y1105) 抗体 (ab55339)

製品の概要

  • 製品名
    Anti-GRLF1 (phospho Y1105) antibody
    GRLF1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GRLF1 (phospho Y1105)
  • 特異性
    This antibody detects endogenous levels of GRLF1 only when phosphorylated at tyrosine 1105.
  • アプリケーション
    適用あり: IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide (Human)(Peptide available as ab106616.)

  • ポジティブ・コントロール
    • IHC-P: Human breast carcinoma tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitopespecific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab55339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

ターゲット情報

  • 機能
    Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor.
  • 配列類似性
    Contains 4 FF domains.
    Contains 1 Rho-GAP domain.
  • 翻訳後修飾
    Tyrosine phosphorylated.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ARHGAP35 antibody
    • GAP associated protein p190 antibody
    • Glucocorticoid receptor DNA binding factor 1 antibody
    • Glucocorticoid receptor DNA-binding factor 1 antibody
    • Glucocorticoid receptor repression factor 1 antibody
    • GRF 1 antibody
    • GRF-1 antibody
    • GRF1 antibody
    • GRLF 1 antibody
    • GRLF1 antibody
    • GRLF1_HUMAN antibody
    • KIAA1722 antibody
    • MGC10745 antibody
    • p190 A antibody
    • p190-A antibody
    • P190A antibody
    • P190A, rat, homolog of antibody
    • p190ARhoGAP antibody
    • p190RhoGAP antibody
    • Rho GAP p190A antibody
    • Rho GTPase-activating protein 35 antibody
    see all

画像

  • ab55339, at 1/50 dilution, staining GRLF1 in paraffin embedded human brain tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising phosphopeptide.

プロトコール

参考文献

ab55339 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (kidney)
Loading amount
60 µg
Specification
kidney
Gel Running Conditions
Reduced Denaturing (7,5%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Sep 07 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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