Anti-GRLF1 抗体 [2389D3a] (ab58674)
Key features and details
- Mouse monoclonal [2389D3a] to GRLF1
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
製品の概要
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製品名
Anti-GRLF1 antibody [2389D3a]
GRLF1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2389D3a] to GRLF1 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Recombinant fragment -
免疫原
Recombinant fragment of Human GRLF1
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.0225% Potassium chloride, 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 0.812% Sodium chloride, 1% BSA -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
ab58674 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
ポリ/モノ
モノクローナル -
クローン名
2389D3a -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab58674の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
特記事項 |
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WB: Use at an assay dependent dilution. Detects a band of approximately 35 kDa for the recombinant fragment (predicted molecular weight for the full length protein: 172 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor. -
配列類似性
Contains 4 FF domains.
Contains 1 Rho-GAP domain. -
翻訳後修飾
Tyrosine phosphorylated.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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別名
- ARHGAP35 antibody
- GAP associated protein p190 antibody
- Glucocorticoid receptor DNA binding factor 1 antibody
see all
画像
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Anti-GRLF1 antibody [2389D3a] (ab58674) + immunising recombinant protein
Predicted band size: 172 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (0)
ab58674 は論文での使用が確認できていません。