Anti-GRK5 抗体 (ab64943)
Key features and details
- Rabbit polyclonal to GRK5
- Suitable for: ELISA, IHC-Fr, WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-GRK5 antibody -
製品の詳細
Rabbit polyclonal to GRK5 -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, IHC-Fr, WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide from internal of human GRK5.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64943の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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IHC-Fr | (1) |
Use at an assay dependent concentration.
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WB | (2) |
1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
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ICC/IF |
Use a concentration of 1 µg/ml.
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
ICC/IF
Use a concentration of 1 µg/ml. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Specifically phosphorylates the activated forms of G protein-coupled receptors. -
組織特異性
Highest levels in heart, placenta, lung > skeletal muscle > brain, liver, pancreas > kidney. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 1 RGS domain. - Information by UniProt
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参照データベース
- Entrez Gene: 2869 Human
- Entrez Gene: 14773 Mouse
- Entrez Gene: 59075 Rat
- Omim: 600870 Human
- SwissProt: P34947 Human
- SwissProt: Q8VEB1 Mouse
- SwissProt: Q62833 Rat
- Unigene: 524625 Human
see all -
別名
- FLJ39780 antibody
- FP2025 antibody
- G protein coupled receptor kinase 5 antibody
see all
画像
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All lanes : Anti-GRK5 antibody (ab64943) at 1/500 dilution
Lane 1 : HUVEC extract
Lane 2 : HepG2 cell extract
Lane 3 : HeLa cell extract
Lane 4 : HeLa cell extract with immunising peptide
Predicted band size: 68 kDa
Observed band size: 68 kDa
Additional bands at: 100 kDa (possible non-specific binding), 40 kDa (possible non-specific binding) -
ICC/IF image of ab64943 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64943, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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IHC image of ab64943 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64943, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab64943 は 5 報の論文で使用されています。
- Marzano F et al. Genetic Catalytic Inactivation of GRK5 Impairs Cardiac Function in Mice Via Dysregulated P53 Levels. JACC Basic Transl Sci 7:366-380 (2022). PubMed: 35540100
- Acharya S et al. β-Arrestin1 and GPCR kinase2 play permissive roles in Src-mediated endocytosis of α4β2 nicotinic ACh receptors. Br J Pharmacol 178:3498-3516 (2021). PubMed: 33844281
- Zhao TL et al. GRK5 promotes tumor progression in renal cell carcinoma. Neoplasma 66:261-270 (2019). PubMed: 30784280
- Funk AJ et al. Increased G protein-coupled receptor kinase (GRK) expression in the anterior cingulate cortex in schizophrenia. Schizophr Res 159:130-5 (2014). PubMed: 25153362
- Rotermund C et al. Diet-induced obesity accelerates the onset of terminal phenotypes in a-synuclein transgenic mice. J Neurochem 131:848-58 (2014). PubMed: 24995537