製品の概要

  • 製品名Anti-GRK3 antibody
    GRK3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GRK3
  • 特異性Targets C terminal of GRK3. The antibody might crossreact with GRK2 (immunogen sequence is 100% homolog with GRK2), but we do not have any testing data showing cross reactivity with GRK2.
  • アプリケーション適用あり: ELISA, WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human, Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH and selected from the C terminal region of GRK3 (Human)

  • ポジティブ・コントロール
    • Mouse heart tissue and SK OV 3 cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab38294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
WB 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 19956569
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors.
  • 配列類似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADRBK2 antibody
    • Adrenergic, beta, receptor kinase 2 antibody
    • ARBK2_HUMAN antibody
    • BARK2 antibody
    • Beta adrenergic receptor kinase 2 antibody
    • Beta ARK 2 antibody
    • Beta-adrenergic receptor kinase 2 antibody
    • Beta-ARK-2 antibody
    • EC 2.7.11.15 antibody
    • G protein coupled receptor kinase 3 antibody
    • G-protein-coupled receptor kinase 3 antibody
    • GRK3 antibody
    see all

Anti-GRK3 antibody 画像

  • Anti-GRK3 antibody (ab38294)

    Predicted band size : 80 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab38294 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38294, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-GRK3 antibody (ab38294) 使用論文

This product has been referenced in:
  • McCormick PJ  et al. Impaired recruitment of Grk6 and beta-Arrestin 2 causes delayed internalization and desensitization of a WHIM syndrome-associated CXCR4 mutant receptor. PLoS One 4:e8102 (2009). WB, ICC/IF ; Human . Read more (PubMed: 19956569) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - whole (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Dec 22 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"