Anti-GRK2 (phospho S29) 抗体 (ab58520)

製品の概要

  • 製品名Anti-GRK2 (phospho S29) antibody
    GRK2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GRK2 (phospho S29)
  • 特異性This antibody detects endogenous levels of GRK2 only when phosphorylated at serine 29.
  • アプリケーション適用あり: IHC-FoFr, IHC-P, ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthesized phosphopeptide derived from human GRK2 around the phosphorylation site of serine 29 (R-A-SP-K-K).

  • ポジティブ・コントロール
    • IHC-P: human breast carcinoma tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab58520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr 1/1000.
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
WB 1/500 - 1/1000. Predicted molecular weight: 80 kDa.

ターゲット情報

  • 機能Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.
  • 組織特異性Expressed in peripheral blood leukocytes.
  • 配列類似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADRBK1 antibody
    • Adrenergic beta receptor kinase 1 antibody
    • ARBK1_HUMAN antibody
    • BARK antibody
    • BARK1 antibody
    • Beta adrenergic receptor kinase 1 antibody
    • Beta ARK 1 antibody
    • Beta ARK1 antibody
    • Beta-adrenergic receptor kinase 1 antibody
    • Beta-ARK-1 antibody
    • FLJ16718 antibody
    • G protein coupled receptor kinase 2 antibody
    • G-protein coupled receptor kinase 2 antibody
    • GRK2 antibody
    see all

Anti-GRK2 (phospho S29) antibody 画像

  • This image shows human breast carcinoma tissue stained with ab58520 at a 1/50 dilution. Right hand image: tissue treated with the immunising phosphopeptide. Left hand image; without the immunising phosphopeptide.
  • Immunohistochemical analysis of adult mouse brain tissue, staining GRK2 (phospho S29) with ab58520.

    Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100and blocked with 10% horse serum for 1 hour at 20°C;. Samples were incubated with primary antibody (1/1000 in 10% horse serum in PBS + 0.1% Triton X-100) for 16 hours at 4°C. An AlexaFluor®488-conjugated donkey anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-GRK2 (phospho S29) antibody (ab58520)

    Lane 1 : Jurkat cells treated with EGF
    Lane 2 : Jurkat cells treated with EGF with phospho peptide


    Predicted band size : 80 kDa

Anti-GRK2 (phospho S29) antibody (ab58520) 使用論文

ab58520 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (adult mouse brain)
Specification adult mouse brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - 0.1% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Dr. Hai-Ying Mary Cheng

Verified customer

投稿 Dec 17 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"