製品の概要

  • 製品名Anti-GRIP1 antibody
    GRIP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GRIP1
  • 特異性This antibody is raised against glutamate receptor-interacting protein 1
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1050 to the C-terminus of Mouse GRIP1.

    (Peptide available as ab27748.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Mouse Brain Tissue Lysate - postnatal day 7, Rat Brain Tissue Lysate - normal adult tissue, Rat Brain Tissue Lysate - postnatal day 7.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab25963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 135 kDa (predicted molecular weight: 122 kDa).

Abcam recommends using BSA as the blocking agent.

ターゲット情報

  • 機能May play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons.
  • 配列類似性Contains 7 PDZ (DHR) domains.
  • ドメインPDZ 6 mediates interaction with the PDZ recognition motif of EFNB1 and EPHB2 and with the C-terminus of PPFIA1 and PPFIA4. PDZ 4 and PDZ 5 mediate interaction with the C-terminus of GRIA2 and GRIA3. PDZ 4, PDZ 5 and PDZ 6 mediate homomultimers. PDZ 7 mediates interaction with PDZ domain of GRASP1. PDZ 7 domain binds CSPG4. PDZ 6 mediates interaction with the C-terminus of liprins-alpha. PDZ 1, PDZ 2 and PDZ 3 mediate interaction with the PDZ-binding motif of FRAS1 (By similarity). PDZ 4 and PDZ 5 mediate interaction with PRLHR.
  • 細胞内局在Cytoplasmic vesicle. Endoplasmic reticulum. Cell junction > synapse > postsynaptic cell membrane. Cytoplasmic and membrane-associated with vesicles, peri-Golgi complexes and endoplasmic reticulum. Enriched in post-synaptic plasma membrane and post-synaptic densities.
  • Information by UniProt
  • 参照データベース
  • 別名
    • as GRIP antibody
    • Glutamate receptor-interacting protein 1 antibody
    • GRIP-1 antibody
    • Grip1 antibody
    • GRIP1_HUMAN antibody
    see all

Anti-GRIP1 antibody 画像

  • All lanes : Anti-GRIP1 antibody (ab25963) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate - postnatal day 7
    Lane 2 : Brain (Rat) Tissue Lysate - normal adult tissue
    Lane 3 : Brain (Rat) Tissue Lysate - postnatal day 7

    Lysates/proteins at 15 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 122 kDa
    Observed band size : 135 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 108 kDa,38 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab25963 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • ab25963 staining GRIP1 (red) in mouse skeletal (gastrocnemius) muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in PBS + Tween-20) for 12 hours at 4°C. A Cy3®-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. DAPI (blue) - Nuclei.

    See Abreview

Anti-GRIP1 antibody (ab25963) 使用論文

ab25963 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Mouse Cell (skeletal muscle)
Specification skeletal muscle
Permeabilization No
Fixative Methanol
Username

Abcam user community

Verified customer

投稿 Sep 12 2014

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain sections)
Specification Brain sections
Username

Dr. Sophie Pezet

Verified customer

投稿 Aug 03 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"