製品の概要

  • 製品名Anti-GRIK2 antibody [EPR6307]
    GRIK2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR6307] to GRIK2
  • アプリケーション適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC/IF or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to residues in the extracellular domain of Human GRIK2.

  • ポジティブ・コントロール
    • Human fetal brain, Human kidney, and A431 lysates; Human kidney, brain and colon tissues.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EPR6307
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab124702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).Can be blocked with GRIK2 peptide (ab169992).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 追加情報Is unsuitable for Flow Cyt,ICC/IF or IP.
  • ターゲット情報

    • 機能Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. Modulates cell surface expression of NETO2.
    • 組織特異性Expression is higher in cerebellum than in cerebral cortex.
    • 関連疾患Defects in GRIK2 are the cause of mental retardation autosomal recessive type 6 (MRT6) [MIM:611092]. It is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic mental retardation. MRT6 patients display mild to severe mental retardation and psychomotor development delay in early childhood. Patients do not have neurologic problems, congenital malformations, or facial dysmorphism. Body height, weight, and head circumference are normal.
    • 配列類似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK2 subfamily.
    • 翻訳後修飾Sumoylation mediates kainate receptor-mediated endocytosis and regulates synaptic transmission. Sumoylation is enhanced by PIAS3 and desumoylated by SENP1.
      Ubiquitinated. Ubiquitination regulates the GRIK2 levels at the synapse by leading kainate receptor degradation through proteasome.
    • 細胞内局在Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • bA487F5.1 antibody
      • EAA4 antibody
      • Excitatory amino acid receptor 4 antibody
      • GLR 6 antibody
      • GLR6 antibody
      • GluK2 antibody
      • GLUK6 antibody
      • GLUR 6 antibody
      • GluR-6 antibody
      • GluR6 antibody
      • Glutamate receptor 6 antibody
      • Glutamate receptor antibody
      • glutamate receptor form A antibody
      • glutamate receptor form B antibody
      • glutamate receptor form C antibody
      • glutamate receptor form D antibody
      • glutamate receptor form E antibody
      • Glutamate receptor ionotropic kainate 2 antibody
      • GRIK 2 antibody
      • GRIK2 antibody
      • GRIK2 protein antibody
      • GRIK2_HUMAN antibody
      • ionotropic kainate 2 antibody
      • MRT6 antibody
      see all

    Anti-GRIK2 antibody [EPR6307] 画像

    • All lanes : Anti-GRIK2 antibody [EPR6307] (ab124702) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Human kidney lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 103 kDa
    • ab124702, at 1/250 dilution, staining GRIK2 in paraffin-embedded Human brain tissue by immunohistochemistry.
    • ab124702, at 1/250 dilution, staining GRIK2 in paraffin-embedded Human colon tissue by immunohistochemistry.
    • ab124702, at 1/250 dilution, staining GRIK2 in paraffin-embedded Human kidney tissue by immunohistochemistry.

    Anti-GRIK2 antibody [EPR6307] (ab124702) 使用論文

    This product has been referenced in:

    See 1 Publication for this product

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    Thank you for contacting us.

    This peptide has been added to the catalog as ab169992 and is now available for order for $166.


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