製品の概要

  • 製品名Anti-GRIK2 antibody
    GRIK2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GRIK2
  • 特異性GluR6 antibody detects endogenous levels of total GluR6 protein.
  • アプリケーション適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human GRIK2 (C terminal).

  • ポジティブ・コントロール
    • Mouse brain extracts; Paraffin-embedded human brain tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/300 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
ELISA 1/5000.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 5 µg/ml.

ターゲット情報

  • 機能Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. Modulates cell surface expression of NETO2.
  • 組織特異性Expression is higher in cerebellum than in cerebral cortex.
  • 関連疾患Defects in GRIK2 are the cause of mental retardation autosomal recessive type 6 (MRT6) [MIM:611092]. It is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic mental retardation. MRT6 patients display mild to severe mental retardation and psychomotor development delay in early childhood. Patients do not have neurologic problems, congenital malformations, or facial dysmorphism. Body height, weight, and head circumference are normal.
  • 配列類似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK2 subfamily.
  • 翻訳後修飾Sumoylation mediates kainate receptor-mediated endocytosis and regulates synaptic transmission. Sumoylation is enhanced by PIAS3 and desumoylated by SENP1.
    Ubiquitinated. Ubiquitination regulates the GRIK2 levels at the synapse by leading kainate receptor degradation through proteasome.
  • 細胞内局在Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bA487F5.1 antibody
    • EAA4 antibody
    • Excitatory amino acid receptor 4 antibody
    • GLR 6 antibody
    • GLR6 antibody
    • GluK2 antibody
    • GLUK6 antibody
    • GLUR 6 antibody
    • GluR-6 antibody
    • GluR6 antibody
    • Glutamate receptor 6 antibody
    • Glutamate receptor antibody
    • glutamate receptor form A antibody
    • glutamate receptor form B antibody
    • glutamate receptor form C antibody
    • glutamate receptor form D antibody
    • glutamate receptor form E antibody
    • Glutamate receptor ionotropic kainate 2 antibody
    • GRIK 2 antibody
    • GRIK2 antibody
    • GRIK2 protein antibody
    • GRIK2_HUMAN antibody
    • ionotropic kainate 2 antibody
    • MRT6 antibody
    see all

Anti-GRIK2 antibody 画像

  • All lanes : Anti-GRIK2 antibody (ab53092) at 1/300 dilution

    Lane 1 : Mouse brain extracts
    Lane 2 : Mouse brain extracts with immunizing peptide


    Predicted band size : 95 kDa
    Observed band size : 95 kDa
  • Immunohistochemical analysis of paraffin-embedded human brain tissue using ab53092 at 1/50 dilution.
  • ICC/IF image of ab53092 stained SHSY5Y cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53092, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.

Anti-GRIK2 antibody (ab53092) 使用論文

ab53092 has not yet been referenced specifically in any publications.

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