製品の概要

  • 製品名Anti-GRF2 (phospho Y504) antibody
    GRF2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GRF2 (phospho Y504)
  • 特異性ab74068 detects endogenous levels of GRF2 only when phosphorylated at tyrosine 504 (Human: Tyr504; Mouse: Tyr514).
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human GRF2 around the phosphorylation site of tyrosine 504 (V-P-YP-A-P)

  • ポジティブ・コントロール
    • Extracts from HepG2 cells treated with Na3VO4 (0.3nM, 40mins).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab74068 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab74068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 121 kDa (predicted molecular weight: 121 kDa).
ELISA 1/10000.

ターゲット情報

  • 機能Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS.
  • 組織特異性Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver.
  • 配列類似性Contains 1 N-terminal Ras-GEF domain.
    Contains 1 Ras-GEF domain.
  • Information by UniProt
  • 参照データベース
  • 製品の状態It localizes to cytoplasm and endosomes.
  • 別名
    • C3G antibody
    • C3G protein antibody
    • CRK SH3 binding GNRP antibody
    • CRK SH3-binding GNRP antibody
    • DKFZp781P1719 antibody
    • GRF 2 antibody
    • GRF2 antibody
    • Guanine nucleotide releasing factor 2 (specific for crk proto oncogene) antibody
    • Guanine nucleotide releasing factor 2 antibody
    • Guanine nucleotide-releasing factor 2 antibody
    • OTTHUMP00000064558 antibody
    • Protein C3G antibody
    • Rap guanine nucleotide exchange factor (GEF) 1 antibody
    • Rap guanine nucleotide exchange factor 1 antibody
    • RAPGEF 1 antibody
    • RAPGEF1 antibody
    • RPGF1_HUMAN antibody
    see all

Anti-GRF2 (phospho Y504) antibody 画像

  • All lanes : Anti-GRF2 (phospho Y504) antibody (ab74068) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells treated with Na3VO4 (0.3nM, 40mins),
    Lane 2 : Extracts from HepG2 cells treated with Na3VO4 (0.3nM, 40mins) with immunising phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 121 kDa
    Observed band size : 121 kDa

Anti-GRF2 (phospho Y504) antibody (ab74068) 使用論文

ab74068 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"