製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GREB1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide within residues: DNGHNALLGF SGNCVGCGKK GFCYFTEFSN HINLKLTTQP KKQKHLKYYL , corresponding to internal sequence amino acids 144-193 of Human GREB1(NP_683701)

  • ポジティブ・コントロール
    • MCF7 cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab84025 is purified by a peptide affinity chromatography method.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab84025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Unknown. Primary target for estrogen receptor regulation. Acts as a regulator of hormone-dependent cancer growth in breast and prostate cancers.
  • 組織特異性
    Expressed in proliferating prostatic tissue and prostate cancer.
  • 配列類似性
    Belongs to the GREB1 family.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5730583K22Rik antibody
    • 9130004E13 antibody
    • AF180470 antibody
    • AU023194 antibody
    • Gene regulated by estrogen in breast cancer protein antibody
    • Gene regulated in breast cancer 1 protein antibody
    • GREB 1 antibody
    • Greb1 antibody
    • GREB1_HUMAN antibody
    • KIAA0575 antibody
    • mKIAA0575 antibody
    • OTTHUMP00000180581 antibody
    • Protein GREB1 antibody
    see all

画像

  • Anti-GREB1 antibody (ab84025) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 43 kDa
    Observed band size : 43 kDa

プロトコール

参考文献

ab84025 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Tissue lysate - whole (brain)
Total protein in input
800 µg
Specification
brain
Immuno-precipitation step
Protein A/G
Username

Abcam user community

Verified customer

投稿 Dec 25 2009

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Jing-Ping Zhang

Verified customer

投稿 Dec 18 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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