製品の概要

  • 製品名Anti-GREB1 antibody
    GREB1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GREB1
  • アプリケーション適用あり: ICC/IF, WB, ChIP, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human GREB1.

    (Peptide available as ab92716.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in human prostate tissue lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal prostate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 216 kDa (predicted molecular weight: 216 kDa).
ChIP Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Unknown. Primary target for estrogen receptor regulation. Acts as a regulator of hormone-dependent cancer growth in breast and prostate cancers.
  • 組織特異性Expressed in proliferating prostatic tissue and prostate cancer.
  • 配列類似性Belongs to the GREB1 family.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5730583K22Rik antibody
    • 9130004E13 antibody
    • AF180470 antibody
    • AU023194 antibody
    • Gene regulated by estrogen in breast cancer protein antibody
    • Gene regulated in breast cancer 1 protein antibody
    • GREB 1 antibody
    • Greb1 antibody
    • GREB1_HUMAN antibody
    • KIAA0575 antibody
    • mKIAA0575 antibody
    • OTTHUMP00000180581 antibody
    • Protein GREB1 antibody
    see all

Anti-GREB1 antibody 画像

  • Anti-GREB1 antibody (ab72999) at 1 µg/ml + Human Prostate Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 216 kDa
    Observed band size : 216 kDa


    Exposure time : 2 minutes
  • ICC/IF image of ab72999 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72999, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • IHC image of GREB1 staining in Human normal prostate formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72999, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-GREB1 antibody (ab72999) 使用論文

This product has been referenced in:
  • Zhang P  et al. Endometrial cancer-associated mutants of SPOP are defective in regulating estrogen receptor-a protein turnover. Cell Death Dis 6:e1687 (2015). Read more (PubMed: 25766326) »
  • Spanheimer PM  et al. Inhibition of RET increases the efficacy of antiestrogen and is a novel treatment strategy for luminal breast cancer. Clin Cancer Res 20:2115-25 (2014). WB . Read more (PubMed: 24526731) »

See all 3 Publications for this product

Product Wall

Application ChIP on chip
Detection step Microarray
Sample Human Cell lysate - nuclear (Chip-Seq in MCF7 cells)
Specification Chip-Seq in MCF7 cells
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Positive control Chip-seq of GREB1 was performed on asynchronous MCF7 cells crosslinked using formaldehyde. 10ug of DNA was used for each experiment. ChIP DNA was then extracted and amplified using Illumina sequencing adapters and DNA was sequenced.
Username

Abcam user community

Verified customer

投稿 Mar 12 2014

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (MCF7 cells)
Specification MCF7 cells
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X-100 solution for 5 min
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: .5%
Username

Abcam user community

Verified customer

投稿 Feb 25 2013

Thank you for your enquiry regardingab72999 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer ishaving problems with this antibody.



Could you please specify what s...

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