Anti-GRC5 / PHF2 抗体 - Carboxyterminal end (ab65771)

製品の概要

  • 製品名Anti-GRC5 / PHF2 antibody - Carboxyterminal end
    GRC5 / PHF2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GRC5 / PHF2 - Carboxyterminal end
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide, corresponding to C terminal amino acids 830-1096 of Human GRC5/ PHF2

  • ポジティブ・コントロール
    • Nuclear extracts of HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab65771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 121 kDa).
IP Use at an assay dependent dilution.

ターゲット情報

  • 機能Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA.
  • 組織特異性Widely expressed, including in liver (at protein level).
  • 配列類似性Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
    Contains 1 JmjC domain.
    Contains 1 PHD-type zinc finger.
  • ドメインThe PHD-type zinc finger mediates the binding to H3K4me2 and H3K4me3.
  • 翻訳後修飾Phosphorylated by PKA on specific serine residues, leading to the formation of an active lysine demethylase complex.
  • 細胞内局在Nucleus > nucleolus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CENP-35 antibody
    • GRC5 antibody
    • JHDM1E antibody
    • Jumonji C domain containing histone demethylase 1E antibody
    • Lysine-specific demethylase PHF2 antibody
    • PHD finger protein 2 antibody
    • Phf2 antibody
    • PHF2_HUMAN antibody
    see all

Anti-GRC5 / PHF2 antibody - Carboxyterminal end 画像

  • Anti-GRC5 / PHF2 antibody - Carboxyterminal end (ab65771) at 1/1000 dilution + Nuclear extracts of HeLa cells.

    Predicted band size : 121 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)

Anti-GRC5 / PHF2 antibody - Carboxyterminal end (ab65771) 使用論文

ab65771 has not yet been referenced specifically in any publications.

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Thank you for contacting us and for your patience while I have been in touch with the lab regarding your enquiry.
You are correct that there was a mistake on the datasheet. The immunogen runs from amino acid 830 to the C-terminus at amino acid 109...

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