製品の概要

  • 製品名Anti-GRC5 / PHF2 antibody
    GRC5 / PHF2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GRC5 / PHF2
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Dog, Pig, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human GRC5/ PHF2.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in both Human and Mouse Liver tissue lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 155 kDa (predicted molecular weight: 120 kDa).

ターゲット情報

  • 機能Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA.
  • 組織特異性Widely expressed, including in liver (at protein level).
  • 配列類似性Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
    Contains 1 JmjC domain.
    Contains 1 PHD-type zinc finger.
  • ドメインThe PHD-type zinc finger mediates the binding to H3K4me2 and H3K4me3.
  • 翻訳後修飾Phosphorylated by PKA on specific serine residues, leading to the formation of an active lysine demethylase complex.
  • 細胞内局在Nucleus > nucleolus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CENP-35 antibody
    • GRC5 antibody
    • JHDM1E antibody
    • Jumonji C domain containing histone demethylase 1E antibody
    • Lysine-specific demethylase PHF2 antibody
    • PHD finger protein 2 antibody
    • Phf2 antibody
    • PHF2_HUMAN antibody
    see all

Anti-GRC5 / PHF2 antibody 画像

  • All lanes : Anti-GRC5 / PHF2 antibody (ab124434) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Liver (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 120 kDa
    Observed band size : 155 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 52 kDa,90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

Anti-GRC5 / PHF2 antibody (ab124434) 使用論文

ab124434 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"