製品の概要

  • 製品名Anti-GRB10 (phospho Y67) antibody
    GRB10 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GRB10 (phospho Y67)
  • 特異性ab61795 detects endogenous levels of GRB 10 only when phosphorylated at tyrosine 67.
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic phosphopeptide derived from human GRB 10 around the phosphorylation site of tyrosine 67 (S-L-YP-S-A).

  • ポジティブ・コントロール
    • NIH 3T3 cell extracts treated with Insulin (0.01U/ml, 15mins).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab61795 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab61795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).

ターゲット情報

  • 機能Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.
  • 組織特異性Widely expressed in fetal and adult tissues, including fetal and postnatal liver, lung, kidney, skeletal muscle, heart, spleen, skin and brain.
  • 配列類似性Belongs to the GRB7/10/14 family.
    Contains 1 PH domain.
    Contains 1 Ras-associating domain.
    Contains 1 SH2 domain.
  • ドメインThe PH domain binds relatively non-specifically to several phosphoinositides, including PI(5)P, PI(4,5)P2, PI(3,4)P2 and PI(3,4,5)P3, with modest affinities.
  • 翻訳後修飾phosphorylated on serine residues upon EGF, FGF and PDGF stimulation.
  • 細胞内局在Cytoplasm. When complexed with NEDD4 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment.
  • Information by UniProt
  • 参照データベース
  • 別名
    • grb 10 antibody
    • GRB IR antibody
    • grb-10 antibody
    • GRB10 adapter protein antibody
    • GRB10 adaptor protein antibody
    • GRB10 antibody
    • GRB10_HUMAN antibody
    • GRBIR antibody
    • Growth factor receptor bound protein 10 antibody
    • Growth factor receptor-bound protein 10 antibody
    • Insulin receptor binding protein antibody
    • Insulin receptor binding protein GRB IR antibody
    • Insulin receptor-binding protein Grb-IR antibody
    • IRBP antibody
    • KIAA0207 antibody
    • Maternally expressed gene 1 antibody
    • MEG1 antibody
    • RSS antibody
    see all

Anti-GRB10 (phospho Y67) antibody 画像

  • All lanes : Anti-GRB10 (phospho Y67) antibody (ab61795) at 1/500 dilution

    Lane 1 : NIH 3T3 cell extracts treated with Insulin (0.01U/ml, 15mins)
    Lane 2 : NIH 3T3 cell extracts treated with Insulin (0.01U/ml, 15mins) with immunising peptide


    Predicted band size : 67 kDa
    Observed band size : 67 kDa

Anti-GRB10 (phospho Y67) antibody (ab61795) 使用論文

ab61795 has not yet been referenced specifically in any publications.

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