Anti-CSP-C 抗体 (ab69876)
Key features and details
- Rabbit polyclonal to CSP-C
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CSP-C antibody
CSP-C 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CSP-C -
由来種
Rabbit -
特異性
This antibody detects endogenous levels of total GZMH protein. -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human CSP-C (internal sequence).
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ポジティブ・コントロール
- A549 cells and extracts from K562 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69876の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | ||
ICC/IF |
特記事項 |
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I-ELISA: 1/5000.
WB: 1/500 - 1/1000. Detects bands of approximately 21 and 54 kDa (predicted molecular weight: 27 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Cytotoxic chymotrypsin-like serine protease with preference for bulky and aromatic residues at the P1 position and acidic residues at the P3' and P4' sites. Probably necessary for target cell lysis in cell-mediated immune responses. Participates in the antiviral response via direct cleavage of several proteins essential for viral replication. -
組織特異性
Constituvely expressed in NK cells. -
配列類似性
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
細胞内局在
Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes. - Information by UniProt
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参照データベース
- Entrez Gene: 2999 Human
- Omim: 116831 Human
- SwissProt: P20718 Human
- Unigene: 348264 Human
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別名
- Cathepsin G like 2 antibody
- Cathepsin G-like 2 antibody
- CCP X antibody
see all
画像
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All lanes : Anti-CSP-C antibody (ab69876) at 1/500 dilution
Lane 1 : Extracts from K562 cells
Lane 2 : Extracts from K562 cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 27 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Additional bands at: 54 kDa. We are unsure as to the identity of these extra bands. -
Immunofluorescence analysis of A549 cells using ab69876 at 1/500-1/1000 dilution.
Left image un-treated.Right image treated with immunizing peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab69876 は 1 報の論文で使用されています。
- Tang H et al. Granzyme H of cytotoxic lymphocytes is required for clearance of the hepatitis B virus through cleavage of the hepatitis B virus X protein. J Immunol 188:824-31 (2012). WB, ICC/IF ; Human . PubMed: 22156339