製品の概要

  • 製品名Anti-Granzyme H antibody
    Granzyme H 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Granzyme H
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Cat
  • 免疫原

    Synthetic peptide corresponding to Human Granzyme H aa 1-50 (N terminal).
    Sequence:

    MQPFLLLLAFLLTPGAGTEEIIGGHEAKPHSRPYMAFVQFLQEKSRKRCG

  • ポジティブ・コントロール
    • Jurkat Cell Lysate, human kidney tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 27 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:1562500.

ターゲット情報

  • 機能Cytotoxic chymotrypsin-like serine protease with preference for bulky and aromatic residues at the P1 position and acidic residues at the P3' and P4' sites. Probably necessary for target cell lysis in cell-mediated immune responses. Participates in the antiviral response via direct cleavage of several proteins essential for viral replication.
  • 組織特異性Constituvely expressed in NK cells.
  • 配列類似性Belongs to the peptidase S1 family. Granzyme subfamily.
    Contains 1 peptidase S1 domain.
  • 細胞内局在Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cathepsin G like 2 antibody
    • Cathepsin G-like 2 antibody
    • CCP X antibody
    • CCP-X antibody
    • CGL 2 antibody
    • CGL2 antibody
    • CSP C antibody
    • CSP-C antibody
    • CTLA1 antibody
    • CTSGL2 antibody
    • Cytotoxic serine protease C antibody
    • Cytotoxic T lymphocyte associated serine esterase 1 antibody
    • Cytotoxic T lymphocyte proteinase antibody
    • Cytotoxic T-lymphocyte proteinase antibody
    • EC 3.4.21.- antibody
    • GRAH_HUMAN antibody
    • Granzyme H (cathepsin G-like 2, protein h-CCPX) antibody
    • Granzyme H antibody
    • GZMH antibody
    • Protein h CCPX antibody
    see all

Anti-Granzyme H antibody 画像

  • Anti-Granzyme H antibody (ab58852) at 2.5 µg/ml + Jurkat Cell Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000 dilution.

    Predicted band size : 27 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Granzyme H with ab58852 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

Anti-Granzyme H antibody (ab58852) 使用論文

ab58852 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"