The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報Flow Cyt: 1/10 - 1/100. Membrane permeabilization is required for flow cytometry. The use of LeucopermTM is recommended for this purpose. Use 10µl of the suggested working dilution to label 106 cells or 100ul whole blood.
IHC-Fr: Use at an assay dependent dilution. Hsi, B-L. et al. (1986)
IP: Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
配列類似性Belongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain.
細胞内局在Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
Cytotoxic T lymphocyte associated serine esterase 1 antibody
Cytotoxic T lymphocyte proteinase 2 antibody
Cytotoxic T-lymphocyte proteinase 2 antibody
Fragmentin 2 antibody
Granzyme 2 antibody
Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1) antibody
Granzyme B antibody
Human lymphocyte protein antibody
Lymphocyte protease antibody
Protease, serine, B antibody
T cell serine protease 1-3E antibody
T-cell serine protease 1-3E antibody
Anti-Granzyme B antibody [GB11] (ab10912) 使用論文
This product has been referenced in:
Demotte N et al. A galectin-3 ligand corrects the impaired function of human CD4 and CD8 tumor-infiltrating lymphocytes and favors tumor rejection in mice. Cancer Res70:7476-88 (2010).
Read more (PubMed: 20719885) »