Anti-Granzyme B 抗体 [CLB-GB11] (ab103159)
Key features and details
- Mouse monoclonal [CLB-GB11] to Granzyme B
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Granzyme B antibody [CLB-GB11]
Granzyme B 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [CLB-GB11] to Granzyme B -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt (Intra)more details -
種交差性
交差種: Human -
免疫原
Full length native protein (purified) corresponding to Human Granzyme B.
Database link: P10144 -
ポジティブ・コントロール
- Flow Cyt: human peripheral whole blood
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
バッファー
pH: 8.0
Preservative: 0.097% Sodium azide
Constituent: Tris buffered saline -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Purified from cell culture supernatant. Purity > 95% (by SDS-PAGE) -
ポリ/モノ
モノクローナル -
クローン名
CLB-GB11 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab103159の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
Use a concentration of 1 - 4 µg/ml.
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特記事項 |
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Flow Cyt (Intra)
Use a concentration of 1 - 4 µg/ml. |
ターゲット情報
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機能
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. -
配列類似性
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
細胞内局在
Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells. - Information by UniProt
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参照データベース
- Entrez Gene: 3002 Human
- Omim: 123910 Human
- SwissProt: P10144 Human
- Unigene: 1051 Human
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別名
- C11 antibody
- Cathepsin G like 1 antibody
- Cathepsin G-like 1 antibody
see all
画像
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Separation of Granzyme B positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracelluar staining) of human peripheral whole blood using ab103159 at 3 μg/ml.
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Flow cytometry intracellular staining pattern of human peripheral whole blood stained using ab103159 at 3 μg/ml.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab103159 は 3 報の論文で使用されています。
- & A blood atlas of COVID-19 defines hallmarks of disease severity and specificity. Cell 185:916-938.e58 (2022). PubMed: 35216673
- Rouers A et al. Immune cell phenotypes associated with disease severity and long-term neutralizing antibody titers after natural dengue virus infection. Cell Rep Med 2:100278 (2021). PubMed: 34095880
- Mishra A et al. Microbial exposure during early human development primes fetal immune cells. Cell 184:3394-3409.e20 (2021). PubMed: 34077752