Anti-Granzyme B 抗体 [B18.1.] (FITC) (ab17929)


  • 製品名
    Anti-Granzyme B antibody [B18.1.] (FITC)
    Granzyme B 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [B18.1.] to Granzyme B (FITC)
  • 標識
    FITC. Ex: 493nm, Em: 528nm
  • 特異性
    Recognizes human granzyme B.
  • アプリケーション
    適用あり: ICC, Flow Cyt, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 7-227 of Human Granzyme B.

  • 特記事項
    1 test means: 1µl of a 1mg/ml stock solution were used to stain 200,000 cells in a sample volume of 50µl.



Our Abpromise guarantee covers the use of ab17929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.


  • 機能
    This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
  • 配列類似性
    Belongs to the peptidase S1 family. Granzyme subfamily.
    Contains 1 peptidase S1 domain.
  • 細胞内局在
    Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C11 antibody
    • Cathepsin G like 1 antibody
    • Cathepsin G-like 1 antibody
    • CCPI antibody
    • CGL 1 antibody
    • CGL1 antibody
    • CSP B antibody
    • CSPB antibody
    • CTLA-1 antibody
    • CTLA1 antibody
    • CTSGL1 antibody
    • Cytotoxic serine protease B antibody
    • Cytotoxic T lymphocyte associated serine esterase 1 antibody
    • Cytotoxic T lymphocyte proteinase 2 antibody
    • Cytotoxic T-lymphocyte proteinase 2 antibody
    • Fragmentin 2 antibody
    • Fragmentin-2 antibody
    • GRAB_HUMAN antibody
    • Granzyme 2 antibody
    • Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1) antibody
    • Granzyme B antibody
    • Granzyme-2 antibody
    • GranzymeB antibody
    • GRB antibody
    • Gzmb antibody
    • Hlp antibody
    • Human lymphocyte protein antibody
    • Lymphocyte protease antibody
    • Protease, serine, B antibody
    • SECT antibody
    • T cell serine protease 1-3E antibody
    • T-cell serine protease 1-3E antibody
    see all

Anti-Granzyme B antibody [B18.1.] (FITC) 画像

  • Detection of granzyme B in permeabilized human CD8+ T lymphocytes (filled profile). Isotype control in combination with a secondary FITC-labeled antibody is shown as control.
    Method: Permeabilized human T cells (5 x 105) were incubated on ice for 30 min in 50 µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1µg of FITC- labelled ab17929 antibody. After washing in FACS buffer, cells were anlyzed by flow cytometry.

  • Detection of granzyme B in a human CD8+ T cell clone.
    Method: Human T cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washes in PBS, 0.1% BSA, slides were incubated for 1 hr at room temperature (RT) with 20 µg/ml of the non-conjugated form of ab17929 antibody in PBS, 0.1% BSA. After rinsing in PBS, a FITC-conjugated anti-mouse IgG was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope.

  • Detection of recombinant granzyme B. Granzyme B migrates as a 27 kDa species.

    Lane 1: 40 mg of lysate
    Lane 2: 4 mg of lysate
    Lane 3: 0.4 mg of lysate

    Method: Recombinant granzyme B was resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-granzyme B non-conjugated form of antibody ab17929 at 1µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.

Anti-Granzyme B antibody [B18.1.] (FITC) (ab17929) 使用論文

This product has been referenced in:
  • Jovanovic DV  et al. CD101 expression and function in normal and rheumatoid arthritis-affected human T cells and monocytes/macrophages. J Rheumatol 38:419-28 (2011). Flow Cyt ; Human . Read more (PubMed: 21159825) »

See 1 Publication for this product

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Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you d...

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