Anti-Granzyme B 抗体 (ab53097)
Key features and details
- Rabbit polyclonal to Granzyme B
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Granzyme B antibody
Granzyme B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Granzyme B -
由来種
Rabbit -
特異性
Detects endogenous levels of total Granzyme B protein. Due to sequence homology this antibody is predicted to cross-react with granzyme H. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide (Human)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53097の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/300 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
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IHC-P | (3) |
Use a concentration of 50 - 100 µg/ml.
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特記事項 |
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WB
1/300 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
IHC-P
Use a concentration of 50 - 100 µg/ml. |
ターゲット情報
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機能
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. -
配列類似性
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
細胞内局在
Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells. - Information by UniProt
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参照データベース
- Entrez Gene: 3002 Human
- Entrez Gene: 14939 Mouse
- Omim: 123910 Human
- SwissProt: P10144 Human
- SwissProt: P04187 Mouse
- Unigene: 1051 Human
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別名
- C11 antibody
- Cathepsin G like 1 antibody
- Cathepsin G-like 1 antibody
see all
画像
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ab53097 (1/50) staining Granzyme B in paraffin-embbeded human breast carcinoma tissue. Right-hand panel represents a negative control for which ab53097 was pre-incubated with the immunizing peptide.
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All lanes : Anti-Granzyme B antibody (ab53097) at 1/300 dilution
Lane 1 : NIH/3T3 cell extract
Lane 2 : NIH/3T3 cell extract with immunizing peptide
Predicted band size: 28 kDa
Observed band size: 28 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab53097 は 8 報の論文で使用されています。
- Aslam MS et al. Therapeutical Significance of Serpina3n Subsequent Cerebral Ischemia via Cytotoxic Granzyme B Inactivation. Biomed Res Int 2022:1557010 (2022). PubMed: 35677097
- Jhuo CF et al. Attenuation of Tumor Development in Mammary Carcinoma Rats by Theacrine, an Antagonist of Adenosine 2A Receptor. Molecules 26:N/A (2021). PubMed: 34946538
- Sun Z et al. Norepinephrine inhibits the cytotoxicity of NK92-MI cells via the ß2-adrenoceptor/cAMP/PKA/p-CREB signaling pathway. Mol Med Rep 17:8530-8535 (2018). PubMed: 29658580
- Tahta A et al. Assessment of the MRI and Behavioral Test Results in a Focal Cerebral Ischemia-Reperfusion Model in the Rat after Separate and Combined Use of Mouse-Derived Neural Progenitor Cells, Human-Derived Neural Progenitor Cells and Atorvastatin. Turk Neurosurg 28:571-581 (2018). PubMed: 30192361
- Sun Y et al. Effect of ginseng polysaccharides on NK cell cytotoxicity in immunosuppressed mice. Exp Ther Med 12:3773-3777 (2016). PubMed: 28105109
- Mohamedali KA et al. The functionalized human serine protease granzyme B/VEGF121 targets tumor vasculature and ablates tumor growth. Mol Cancer Ther 12:2055-66 (2013). ICC/IF . PubMed: 23858102
- Chaitanya GV et al. Role of cytotoxic protease granzyme-b in neuronal degeneration during human stroke. Brain Pathol 21:16-30 (2011). PubMed: 20825413
- Chaitanya GV et al. Granzyme-b is involved in mediating post-ischemic neuronal death during focal cerebral ischemia in rat model. Neuroscience 165:1203-16 (2010). WB ; Rat . PubMed: 19895873