Anti-Granulocytes 抗体 [HIS48] (ab33760)

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製品の概要

  • 製品名
    Anti-Granulocytes antibody [HIS48]
    Granulocytes 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HIS48] to Granulocytes
  • 由来種
    Mouse
  • 特異性
    ab3760 recognises granulocytes and erythroid cells.
  • アプリケーション
    適用あり: IHC-Fr, Flow Cyt, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Rat
  • 免疫原

    PVG rat spleen cell suspension.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: Tissue culture supernatant, 8% Foetal calf serum, 0.2M Tris HCl. pH 7.4
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    HIS48
  • アイソタイプ
    IgM
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab33760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/20.
Flow Cyt Use 10µl for 106 cells.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

 
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    Granulocytes are a type of white blood cell filled with microscopic granules that are little sacs containing enzymes, compounds that digest microorganisms. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. They do not respond exclusively to specific antigens, as do B-cells and T-cells. Neutrophils, eosinophils and basophils are all types of granulocytes. They are named by the staining features of their granules in the laboratory: Neutrophils have "neutral" subtle granules; Eosinophils have prominent granules that stain readily with the acid dye eosin; and Basophils have prominent granules that stain readily basic (non acidic) dyes.

参考文献

ab33760 has not yet been referenced specifically in any publications.

ab33760 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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4 reviews or Q&A

10
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I need to ask you some questions about antibody ab33760
What epitop are this antibodies specific to? In other words, what proteins/receptors has activated neutrophils, which are staninig by this antybody, It's very important for us because we try ...

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Thank you for contacting us.

The immunogen used for this antibody was not a specific peptide but rather a cell suspension from rat spleen. Unfortunately, we have not performed any detailed mapping on this clone and thus we currently do not h...

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, You mention that the ab33760 is specifically designed against neutrophil and rbc. I would like to know how can you be sure that the ab33760 does not recognize the same antigen present on monocyte or macrophage? Thank you

Thank you for your inquiry. According to our records, the specificity of this clone is supported by the following papers:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1783230/

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2793189/
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Dear John Thank you for your answer. Sorry, I think that my previous question wasn't right. It doesn't matter what sequence it is. I try to understand why this antibody is specific only for granulocytes?

Thank you for your reply. I am sorry that I misunderstood your original email. The antibody ab33760 was generated from PVG rat spleen cell suspension and is specific to granulocytes and erythroid cells. Unfortunately I do not have any more information ...

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Hello We often buy production of your firm and are very happy with received results but now I need to ask you some questions about antibody ab33760 What epitop are this antibodies specific to?

Thank you for contacting Abcam. The antibody ab33760, anti-Granulocytes antibody [HIS48] was generated from PVG rat spleen cell suspensions. The exact epitope sequence is proprietary and I do not have access to that information and therefore I cannot t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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