The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/200.
Use 10µl of the suggested working dilution to label 1x106 cells in 100µl.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
関連性Granulocytes are a type of white blood cell filled with microscopic granules that are little sacs containing enzymes, compounds that digest microorganisms. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. They do not respond exclusively to specific antigens, as do B-cells and T-cells.
Neutrophils, eosinophils and basophils are all types of granulocytes. They are named by the staining features of their granules in the laboratory: Neutrophils have "neutral" subtle granules; Eosinophils have prominent granules that stain readily with the acid dye eosin; and Basophils have prominent granules that stain readily basic (non acidic) dyes.
Anti-Granulocytes antibody [2B2] (ab67418) 使用論文
has not yet been referenced specifically in any publications.