製品の概要

  • 製品名Anti-Granulin antibody
    Granulin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Granulin
  • アプリケーション適用あり: WB, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment: SCEKEVVSAQ PATFLARSPH VGVKDVECGE GHFCHDNQTC CRDNRQGWAC CPYRQGVCCA DRRHCCPAGF RCAARGTKCL RREAPRWDAP LRDPALRQLL , corresponding to amino acids 494-594 of Human Granulin

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 64 kDa.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling.
    Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth.
  • 組織特異性In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney.
  • 関連疾患Defects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD) [MIM:607485]; also known as tau-negative frontotemporal dementia linked to chromosome 17. Frontotemporal dementia (FTD) is the second most common cause of dementia in people under the age of 65 years. It is an autosomal dominant neurodegenerative disease.
  • 配列類似性Belongs to the granulin family.
  • 翻訳後修飾Granulins are disulfide bridged.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Acrogranin antibody
    • CLN11 antibody
    • GEP antibody
    • GP88 antibody
    • Granulin A antibody
    • Granulin B antibody
    • Granulin C antibody
    • Granulin D antibody
    • Granulin E antibody
    • Granulin epithelin antibody
    • Granulin F antibody
    • Granulin G antibody
    • Granulin-7 antibody
    • Granulins antibody
    • GRN antibody
    • GRN_HUMAN antibody
    • PC cell derived growth factor antibody
    • PCDGF antibody
    • PEPI antibody
    • PGRN antibody
    • Proepithelin antibody
    • Progranulin antibody
    see all

Anti-Granulin antibody 画像



  • Predicted band size : 64 kDa
    Granulin antibody (ab55167) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab55167 at 10 ug/ml staining Granulin in human Hela cells by Immunocytochemistry/ Immunofluorescence.

Anti-Granulin antibody (ab55167) 使用論文

ab55167 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Fixation Methanol
Permeabilization Yes - methanol
Sample Human Cell (HELA cells)
Specification HELA cells
Gating Strategy Hela cells were gated for FSC and SSC parameters.
Preparation Cell harvesting/tissue preparation method: Rinse the cell layer with 0.25% (w/v) Trypsin- EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Sample buffer: Trypsin-EDTA solution
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Abcam user community

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投稿 Feb 24 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (LIVER)
Specification LIVER
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Abcam user community

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投稿 Jul 29 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Tissue sections (Brain tissues, fresh frozen)
Specification Brain tissues, fresh frozen
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
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Dr. Ruma Raha-Chowdhury

Verified customer

投稿 May 25 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"