Anti-GRAMD1C 抗体 (ab121365)
Key features and details
- Rabbit polyclonal to GRAMD1C
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-GRAMD1C antibody -
製品の詳細
Rabbit polyclonal to GRAMD1C -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Orangutan -
免疫原
Recombinant fragment, corresponding to amino acids 452-541 of Human GRAMD1C (Q8IYS0).
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ポジティブ・コントロール
- Human smooth muscle tissue; RT4, U251 MG, Human liver and tonsil lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121365の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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特記事項 |
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
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配列類似性
Contains 1 GRAM domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 54762 Human
- Entrez Gene: 100172272 Orangutan
- SwissProt: Q8IYS0 Human
- SwissProt: Q5RC33 Orangutan
- Unigene: 24583 Human
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別名
- 4921521N14Rik antibody
- GRAM domain-containing protein 1C antibody
- GRAMD1C antibody
see all
画像
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Immunofluorescent staining of Human cell line U-251MG shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab121365 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab121365 at 1/500 dilution staining GRAMD1C in Paraffin-emebedded Human Smooth muscle tissue by Immunohistochemistry.
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All lanes : Anti-GRAMD1C antibody (ab121365) at 1/250 dilution
Lane 1 : RT4 lysate
Lane 2 : U251 MG lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab121365 は論文での使用が確認できていません。