Rabbit polyclonal to GPT2
This antibody may cross-react with GPT1.
Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
Synthetic peptide corresponding to a region within C terminal amino acids 360-409 (HPEIKGQLVK LLSVRLCPPV SGQAAMDIVV NPPVAGEESF EQFSREKESV) of Human GPT2 (NP_597700).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use at an assay dependent dilution. ELISA titre using peptide based assay: 1/62500.
Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 58 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate.
Expressed at high levels in muscle, adipose tissue, kidney and brain and at lower levels in the liver and breast.
Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.
Information by UniProt
Alanine aminotransferase 2 antibody
Western blot - Anti-GPT2 antibody (ab80947)
Anti-GPT2 antibody (ab80947) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal brain lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size:
Observed band size:
65 kDa (
why is the actual band size different from the predicted?
Gel concentration: 12%
Immunocytochemistry/ Immunofluorescence - Anti-GPT2 antibody (ab80947)
ICC/IF image of ab80947 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80947, 5µg/ml) overnight at +4°C. The secondary antibody (green) was
, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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