製品の概要

  • 製品名Anti-GPSM3 antibody
  • 製品の詳細
    Mouse monoclonal to GPSM3
  • アプリケーション適用あり: WB, ELISA, Sandwich ELISAmore details
  • 種交差性
    交差種: Recombinant Fragment
    交差が予測される動物種: Human
  • 免疫原

    Recombinant full length Human GPSM3 (amino acids 1-160) with a proprietary tag (AAH18724).

  • ポジティブ・コントロール
    • GPSM3 transfected 293T cell line; GPSM3 Recombinant protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーpH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab118118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 18 kDa. This antibody has only been tested against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ELISA Use at an assay dependent dilution.
Sandwich ELISA Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Interacts with subunit of G(i) alpha proteins and regulates the activation of G(i) alpha proteins.
  • 組織特異性Expressed in heart, placenta, lung and liver.
  • 配列類似性Contains 3 GoLoco domains.
  • ドメインThe GoLoco 1 and/or GoLoco 3 domains exhibit GDI activity towards GDP-bound G(i) alpha protein, but not the GoLoco 2 domain.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Activator of G-protein signaling 4 antibody
    • AGS4 antibody
    • C6orf9 antibody
    • G protein signalling modulator 3 (AGS3 like, C. elegans) antibody
    • G-protein signaling modulator 3 (AGS3-like, C. elegans) antibody
    • G-protein-signaling modulator 3 antibody
    • G18 antibody
    • G18.1a antibody
    • G18.1b antibody
    • G18.2 antibody
    • Gpsm3 antibody
    • GPSM3_HUMAN antibody
    • NG1 antibody
    • OTTHUMP00000029351 antibody
    • Protein G18 antibody
    see all

Anti-GPSM3 antibody 画像

  • Anti-GPSM3 antibody (ab118118) at 5 µg/ml + Recombinant protein at 0.2 µg

    Predicted band size : 18 kDa
  • All lanes : Anti-GPSM3 antibody (ab118118) at 5 µg/ml

    Lane 1 : 293T cell line, transfected with GPSM3
    Lane 2 : 293T cell line, non transfected

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 18 kDa
  • Detection limit for recombinant tagged GPSM3 is 0.3 ng/ml as a capture antibody.

Anti-GPSM3 antibody (ab118118) 使用論文

ab118118 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing (13.5% gel)
Sample Mouse Tissue lysate - whole (Thymus, Spleen, Blood)
Specification Thymus, Spleen, Blood
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 Jun 24 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"