製品の概要

  • 製品名Anti-GPR2/CCR10 antibody
    GPR2/CCR10 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GPR2/CCR10
  • アプリケーション適用あり: IP, ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Sheep, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human GPCR GPR2/CCR10.

    (Peptide available as ab32846.)

  • ポジティブ・コントロール
    • MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab30718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/250. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa).
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能Receptor for chemokines SCYA27 and SCYA28. Subsequently transduces a signal by increasing the intracellular calcium ions level and stimulates chemotaxis in a pre-B cell line.
  • 組織特異性Expressed at high levels in adult testis, small intestine, fetal lung, fetal kidney. Weaker expression was observed in many other adult tissues including spleen, thymus, lymph node, Peyer patches, colon, heart, ovary, peripheral blood lymphocytes, thyroid and spinal cord. Also expressed by melanocytes, dermal fibroblasts, dermal microvascular endothelial cells. Also detected in T-cells and in skin-derived Langerhans cells.
  • 配列類似性Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C C chemokine receptor type 10 antibody
    • C-C chemokine receptor type 10 antibody
    • C-C CKR-10 antibody
    • CC chemokine receptor 10 antibody
    • CC CKR 10 antibody
    • CC-CKR-10 antibody
    • CCR-10 antibody
    • CCR10 antibody
    • CCR10_HUMAN antibody
    • Chemokine (C C motif) receptor 10 antibody
    • G protein coupled receptor 2 antibody
    • G-protein coupled receptor 2 antibody
    • GPR2 antibody
    see all

Anti-GPR2/CCR10 antibody 画像

  • GPR2/CCR10 was immunoprecipitated using 0.5mg MCF7 whole cell extract, 5µg of Rabbit polyclonal to GPR2/CCR10 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, MCF7 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab30718.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 42kDa; GPR2/CCR10

  • Anti-GPR2/CCR10 antibody (ab30718) at 1/250 dilution + MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • Image courtesy of Human Protein Atlas

    ab30718 staining GPCR GPR2/CCR10 in Human smooth muscle. The praffin embedded tissue was incubated with ab30718 (1/2000 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Ab30718 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.

    Further results for this antibody can be found at www.proteinatlas.org

  • ICC/IF image of ab30718 stained human HEK 293 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab30718, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

Anti-GPR2/CCR10 antibody (ab30718) 使用論文

ab30718 has not yet been referenced specifically in any publications.

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