製品の概要

  • 製品名
  • 製品の詳細
    Chicken polyclonal to GPR177
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human GPR177 aa 524-541 (C terminal).
    Sequence:

    HVDGPTEIYKLTRKEAGE

  • ポジティブ・コントロール
    • HEK293 cell lysate transfected with GPR177-GFP. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: SKNSH.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
WB 1/2000 - 1/5000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).

ターゲット情報

  • 機能
    Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development.
  • 配列類似性
    Belongs to the wntless family.
  • 細胞内局在
    Golgi apparatus membrane. Cytoplasmic vesicle membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C1orf139 antibody
    • DKFZp686I0788 antibody
    • Evenness interrupted, Drosophila, homolg of antibody
    • EVI antibody
    • FLJ23091 antibody
    • G protein coupled receptor 177 antibody
    • GPR 177 antibody
    • Integral membrane protein GPR177 antibody
    • MGC131760 antibody
    • MGC14878 antibody
    • mig-14 antibody
    • MRP antibody
    • Protein evenness interrupted homolog antibody
    • Protein wntless homolog antibody
    • Putative NF-kappa-B-activating protein 373 antibody
    • Putative NFkB activating protein 373 antibody
    • WLS antibody
    • WLS_HUMAN antibody
    • wntless homolog (Drosophila) antibody
    • Wntless homolog antibody
    • wntless Wnt ligand secretion mediator antibody
    • Wntless, Drosophila, homolog of antibody
    see all

画像

  • ICC/IF image of ab72385 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab72385 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- chicken (ab96947) IgY (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-GPR177 antibody (ab72385) at 1/2000 dilution

    Lane 1 : Molecular weight markers
    Lane 2 : HEK293 cell lysate transfected with GPR177-GFP


    Predicted band size : 62 kDa
    Observed band size : 62 kDa
    The HEK293 cell protein lysate contains both transfected GPR177-GFP fusion protein (75 kDa) and endogenous GPR177 protein (~62 kDa).

参考文献

This product has been referenced in:
  • de Groot RE  et al. Retromer dependent recycling of the wnt secretion factor wls is dispensable for stem cell maintenance in the Mammalian intestinal epithelium. PLoS One 8:e76971 (2013). WB ; Mouse . Read more (PubMed: 24130821) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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