製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GPNMB
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 181-407 of Human GPNMB (Q14956).

  • ポジティブ・コントロール
    • PC3 whole cell lysate; U87 xenograft tissue. IF/ICC: Malme-3m cell line.

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab125898 is purified by antigen-affinity chromatography.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab125898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB 1/1000 - 1/10000. Predicted molecular weight: 64 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Could be a melanogenic enzyme.
  • 組織特異性
    Not restricted to the melanocytic lineage.
  • 配列類似性
    Belongs to the PMEL/NMB family.
    Contains 1 PKD domain.
  • 発生段階
    Expression in poorly metastatic melanoma cell lines; no expression in highly metastatic melanoma cell lines.
  • 細胞内局在
    Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Glycoprotein (transmembrane) nmb antibody
    • Glycoprotein nmb antibody
    • Glycoprotein nmb like protein antibody
    • Gpnmb antibody
    • GPNMB_HUMAN antibody
    • HGFIN antibody
    • NMB antibody
    • Osteoactivin antibody
    • Transmembrane glycoprotein antibody
    • Transmembrane glycoprotein HGFIN antibody
    • Transmembrane glycoprotein NMB antibody
    see all

画像

  • ab125898 stained Malme-3m cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab125898 at 1µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-GPNMB antibody (ab125898) at 1/5000 dilution + PC-3 whole cell lysate at 30 µg

    Predicted band size : 64 kDa
    7.5% SDS PAGE.
  • ab125898, at 1/500 dilution, staining GPNMB in Formalin-fixed, Paraffin-embedded U87 xenograft tissue by Immunohistochemistry.

参考文献

ab125898 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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