製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GPLD1
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Human GPLD1.

    (Peptide available as ab92422.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human and Mouse Plasma Total Protein Preparations.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab82330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 92 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane.
    • 配列類似性
      Belongs to the GPLD1 family.
      Contains 7 FG-GAP repeats.
    • 細胞内局在
      Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Glycoprotein phospholipase D antibody
      • Glycosyl-phosphatidylinositol-specific phospholipase D antibody
      • GPI-PLD antibody
      • GPI-specific phospholipase D antibody
      • GPIPLD antibody
      • GPIPLDM antibody
      • GPLD1 antibody
      • MGC22590 antibody
      • PHLD_HUMAN antibody
      • Phosphatidylinositol glycan specific phospholipase D antibody
      • Phosphatidylinositol-glycan-specific phospholipase D antibody
      • PI-G PLD antibody
      • PIGPLD antibody
      • PIGPLD1 antibody
      see all

    画像

    • All lanes : Anti-GPLD1 antibody (ab82330) at 1 µg/ml

      Lane 1 : Human Plasma Total Protein Lysate
      Lane 2 : Mouse Plasma Total Protein Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 92 kDa
      Observed band size: 120 kDa (why is the actual band size different from the predicted?)


      Exposure time: 5 minutes


      The GPLD1 protein has a predicted molecular weight of 92 kDa, however it has several potential glycosylation sites (SwissProt data) that might explain its migration at a higher MW. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

    プロトコール

    参考文献

    ab82330 has not yet been referenced specifically in any publications.

    レビューと Q&A

    There are currently no Customer reviews or Questions for ab82330.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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