製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GPCR RDC1
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human GPCR RDC1.

    (Peptide available as ab38088.)

  • ポジティブ・コントロール
    • This antibody gave a positive result with human brain, testis and heart tissue lysates. Human kidney is a suitable positive control for IHC.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab38089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/250. Detects a band of approximately 52 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human GPCR RDC1 peptide (ab38088).

GPCR RCD1 is glycosylated so it is not unexpected that it runs higher than predicted (based on primary structure) in SDS PAGE.

IHC-P 1/100.

ターゲット情報

  • 機能
    Receptor for CXCL12/SDF1. Acts as coreceptor with CXCR4 for a restricted number of HIV isolates.
  • 組織特異性
    Expressed in monocytes, basophils, and B-cells. Lower expression in CD4+ T-lymphocytes and natural killer cells.
  • 配列類似性
    Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ACKR3 antibody
    • atypical chemokine receptor 3 antibody
    • C X C chemokine receptor type 7 antibody
    • C-X-C chemokine receptor type 7 antibody
    • Chemokine (C-X-C motif) receptor 7 antibody
    • Chemokine C X C motif receptor 7 antibody
    • Chemokine orphan receptor 1 antibody
    • CMKOR1 antibody
    • CXC R7 antibody
    • CXC-R7 antibody
    • CXCR 7 antibody
    • CXCR-7 antibody
    • CXCR7 antibody
    • CXCR7_HUMAN antibody
    • G protein coupled receptor 159 antibody
    • G protein coupled receptor antibody
    • G protein coupled receptor RDC1 homolog antibody
    • G-protein coupled receptor 159 antibody
    • G-protein coupled receptor RDC1 homolog antibody
    • GPR159 antibody
    • GPRN1 antibody
    • RDC 1 antibody
    • RDC-1 antibody
    • RDC1 antibody
    see all

Anti-GPCR RDC1 antibody 画像

  • All lanes : Anti-GPCR RDC1 antibody (ab38089) at 1/250 dilution

    Lane 1 : Human Brain Tissue Lysate
    Lane 2 : Human Testis Tissue Lysate
    Lane 3 : Human Liver Tissue Lysate
    Lane 4 : Human Heart Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
  • ab38089 staining pancreas (human and mouse) kidney (mouse and rat) and testis (rat and human) tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in sequential (prediluted) peroxidase and protein block for 20 minutes at 20°C.  The primary antibody was diluted 1/100 and incubated with the samples for 45 minutes at 20°C.  A HRP-conjugated goat anti-rabbit anitbody was used as the secondary.

    See Abreview

Anti-GPCR RDC1 antibody (ab38089) 使用論文

This product has been referenced in:
  • Hsiao JJ  et al. Androgen receptor and chemokine receptors 4 and 7 form a signaling axis to regulate CXCL12-dependent cellular motility. BMC Cancer 15:204 (2015). Read more (PubMed: 25884570) »
  • Walentowicz-Sadlecka M  et al. Stromal derived factor-1 (SDF-1) and its receptors CXCR4 and CXCR7 in endometrial cancer patients. PLoS One 9:e84629 (2014). IHC-P ; Human . Read more (PubMed: 24416254) »

See all 7 Publications for this product

Product Wall

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 23 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa, Jurkat)
Loading amount
100 µg
Specification
HeLa, Jurkat
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 18°C
Username

Mrs. Merly Saare

Verified customer

投稿 Sep 22 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Pancreas (human) kidney and pancreas (mouse) kidne)
Specification
Pancreas (human) kidney and pancreas (mouse) kidne
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate Buffer
Permeabilization
No
Blocking step
sequential (prediluated) peroxidase and protein block as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

投稿 Apr 18 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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