Anti-GPCR RDC1/CXCR-7 抗体 (ab117836)
Key features and details
- Rabbit polyclonal to GPCR RDC1/CXCR-7
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-GPCR RDC1/CXCR-7 antibody
GPCR RDC1/CXCR-7 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GPCR RDC1/CXCR-7 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken, Cow, Dog -
免疫原
Synthetic peptide corresponding to Mouse GPCR RDC1/CXCR-7 aa 300-400 (C terminal) conjugated to keyhole limpet haemocyanin.
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ポジティブ・コントロール
- ICC/IF: HEK293 cells, HeLa cells. WB: Mouse brain lysate, Rat brain lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99% Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab117836の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/5000. Predicted molecular weight: 41 kDa.
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ICC/IF |
1/5000.
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特記事項 |
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WB
1/5000. Predicted molecular weight: 41 kDa. |
ICC/IF
1/5000. |
ターゲット情報
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機能
Receptor for CXCL12/SDF1. Acts as coreceptor with CXCR4 for a restricted number of HIV isolates. -
組織特異性
Expressed in monocytes, basophils, and B-cells. Lower expression in CD4+ T-lymphocytes and natural killer cells. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 509585 Cow
- Entrez Gene: 403964 Dog
- Entrez Gene: 57007 Human
- Entrez Gene: 12778 Mouse
- Entrez Gene: 84348 Rat
- Omim: 610376 Human
- SwissProt: P11613 Dog
- SwissProt: P25106 Human
see all -
別名
- ACKR3 antibody
- atypical chemokine receptor 3 antibody
- C X C chemokine receptor type 7 antibody
see all
画像
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Immunocytochemistry analysis of HEK293 cells using 1/5000 ab117836.
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Immunofluorescence analysis of CXCR7 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CXCR7 Rabbit Polyclonal Antibody (ab117836) at 1/1000 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin (1/300). Panel d is a merged image showing Cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
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All lanes :
Lane 1 : Mouse brain whole cell lysate
Lane 2 : Rat brain whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 41 kDa
Observed band size: 41 kDaThe blots were probed with Anti-CXCR7 Rabbit Polyclonal Antibody (ab117836, 1/4000-1/6000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (0.4 µg/mL, 1/2500 dilution).
Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel, XCell SureLock™ Electrophoresis System and Novex® Sharp Pre-Stained Protein Standard. Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate.
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Anti-GPCR RDC1/CXCR-7 antibody (ab117836) at 1/5000 dilution + Mouse brain lysate
Predicted band size: 41 kDa
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (5)
ab117836 は 5 報の論文で使用されています。
- Li J et al. S100A9-CXCL12 activation in BRCA1-mutant breast cancer promotes an immunosuppressive microenvironment associated with resistance to immunotherapy. Nat Commun 13:1481 (2022). PubMed: 35304461
- Zimowska M et al. IL-4 and SDF-1 Increase Adipose Tissue-Derived Stromal Cell Ability to Improve Rat Skeletal Muscle Regeneration. Int J Mol Sci 21:N/A (2020). PubMed: 32392778
- Archacka K et al. Beneficial Effect of IL-4 and SDF-1 on Myogenic Potential of Mouse and Human Adipose Tissue-Derived Stromal Cells. Cells 9:N/A (2020). PubMed: 32560483
- Chen D et al. Crosstalk between SDF-1/CXCR4 and SDF-1/CXCR7 in cardiac stem cell migration. Sci Rep 5:16813 (2015). WB . PubMed: 26578388
- Brzoska E et al. Sdf-1 (CXCL12) induces CD9 expression in stem cells engaged in muscle regeneration. Stem Cell Res Ther 6:46 (2015). WB, ICC . PubMed: 25890097