製品の概要

  • 製品名Anti-GPCR LGR7 antibody
    GPCR LGR7 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GPCR LGR7
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Chimpanzee, Monkey
    交差が予測される動物種: Chicken, Cow, Dog, Rhesus monkey, Opossum, Orangutan
  • 免疫原

    Synthetic peptide: VDDCGNQADE DNCGDNNGWS LQFDKYFASY YKMTSQYPFE AETPECLVGS V , corresponding to amino acids 50-100 of Human GPCR LGR7

  • ポジティブ・コントロール
    • Human, mouse and rat heart lysates.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).

ターゲット情報

  • 機能Receptor for relaxins. The activity of this receptor is mediated by G proteins leading to stimulation of adenylate cyclase and an increase of cAMP. Binding of the ligand may also activate a tyrosine kinase pathway that inhibits the activity of a phosphodiesterase that degrades cAMP.
  • 組織特異性Expressed in the brain, kidney, testis, placenta, uterus, ovary, adrenal, prostate, skin and heart. Not detected in spleen.
  • 配列類似性Belongs to the G-protein coupled receptor 1 family.
    Contains 1 LDL-receptor class A domain.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 LRRNT domain.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Gm1018 antibody
    • Leucine rich repeat containing G protein coupled receptor 7 antibody
    • Leucine-rich repeat-containing G-protein coupled receptor 7 antibody
    • LGR 7 antibody
    • LGR7 antibody
    • LGR7.1 antibody
    • LGR7.10 antibody
    • LGR7.2 antibody
    • MGC138347 antibody
    • MGC142177 antibody
    • Relaxin family peptide receptor 1 antibody
    • Relaxin receptor 1 antibody
    • Relaxin/insulin like family peptide receptor 1 antibody
    • RXFP 1 antibody
    • RXFP1 antibody
    • RXFP1_HUMAN antibody
    see all

Anti-GPCR LGR7 antibody 画像

  • Lanes 1 - 2 : Anti-GPCR LGR7 antibody (ab72159) at 0.5 µg/ml
    Lane 3 : Anti-GPCR LGR7 antibody (ab72159) at 1 µg/ml
    Lane 4 : Anti-GPCR LGR7 antibody (ab72159) at 2 µg/ml

    Lane 1 : human heart lysate
    Lane 2 : human heart lysate with immunizing peptide
    Lane 3 : mouse heart lysate
    Lane 4 : rat heart lysate


    Predicted band size : 87 kDa
    Observed band size : 87 kDa

Anti-GPCR LGR7 antibody (ab72159) 使用論文

This product has been referenced in:
  • Dehghan F  et al. Changes in Knee Laxity and Relaxin Receptor Isoforms Expression (RXFP1/RXFP2) in the Knee throughout Estrous Cycle Phases in Rodents. PLoS One 11:e0160984 (2016). WB ; Rat . Read more (PubMed: 27513858) »
  • Dehghan F  et al. Testosterone reduces knee passive range of motion and expression of relaxin receptor isoforms via 5a-dihydrotestosterone and androgen receptor binding. Int J Mol Sci 15:4619-34 (2014). WB ; Rat . Read more (PubMed: 24642882) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step None
Sample Rat Tissue sections (brain)
Specification brain
Permeabilization Yes - 0.25 % Triton
Fixative Paraformaldehyde
Username

Dr. Cheryl Heesch

Verified customer

投稿 Oct 17 2013

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I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask...

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Thank you for contacting us and for providing the details of your protocol. I would expect the protocol you provided to give good signal on rat uterus lysates in WB. For our quality investigation, can you confirm that there were no bands at all on the ...

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