The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
機能Receptor for ADP. Coupled to G(i)-proteins. May play a role in hematopoiesis and the immune system.
組織特異性Strong expression in spleen and adult brain. Lower expression in placenta, lung, liver, spinal cord, thymus, small intestine, uterus, stomach, testis, fetal brain, and adrenal gland. Not detected in pancreas, heart, kidney, skeletal muscle, ovary or fetal aorta. Clearly detected in lymph node and bone marrow, weakly detected in peripheral blood mononuclear cells (PBMC) and in peripheral blood leukocytes (PBL), but not detected in polymorphonuclear cells (PMN). In the brain, detected in all brain regions examined.
配列類似性Belongs to the G-protein coupled receptor 1 family.
製品の状態There are 2 isoforms produced by alternative splicing.
G protein coupled receptor 86 antibody
G protein coupled receptor 94 antibody
G-protein coupled receptor 86 antibody
G-protein coupled receptor 94 antibody
P2Y purinoceptor 13 antibody
Purinergic receptor P2Y G protein coupled 13 antibody
Anti-GPCR GPR86 antibody 画像
Western blot - GPCR GPR86 antibody (ab75103)
All lanes : Anti-GPCR GPR86 antibody (ab75103) at 1/500 dilution
Lane 1 : Extracts from HeLa cells Lane 2 : Extracts from COLO cells Lane 3 : Extracts from HT-29 cells Lane 4 : Extracts from HepG2 cells Lane 5 : Extracts from HUVEC cells Lane 6 : Extracts from COLO cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 38 kDa Observed band size : 38 kDa
Anti-GPCR GPR86 antibody (ab75103) 使用論文
This product has been referenced in:
Espelt MV et al. On the role of ATP release, ectoATPase activity, and extracellular ADP in the regulatory volume decrease of Huh-7 human hepatoma cells. Am J Physiol Cell Physiol304:C1013-26 (2013).
Read more (PubMed: 23485713) »